Characterization of P450 FcpC, the Enzyme Responsible for Bioconversion of Diosgenone to Isonuatigenone in Streptomyces Virginiae IBL-14

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2009 Apr 21

PMID 19376895

Citations 7

Authors

Wei Wang

Feng-Qing Wang

Dong-Zhi Wei

Affiliations

Soon will be listed here.

Abstract

A new cytochrome P450 monooxygenase, FcpC, from Streptomyces virginiae IBL-14 has been identified. This enzyme is found to be responsible for the bioconversion of a pyrano-spiro steroid (diosgenone) to a rare nuatigenin-type spiro steroid (isonuatigenone), which is a novel C-25-hydroxylated diosgenone derivative. A whole-cell P450 system was developed for the production of isonuatigenone via the expression of the complete three-component electron transfer chain in an Escherichia coli strain.

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