Induction of Macrophage Plasminogen Activator by Asbestos is Independent of PKC Activation

Overview

Journal Arch Toxicol

Specialty Toxicology

Date 1991 Jan 1

PMID 1929853

Authors

D Lison

F Raguzzi

R Lauwerys

Affiliations

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Abstract

This study was undertaken to assess whether plasminogen activator (PA) induction in macrophages exposed to chrysotile fibers is mediated by protein kinase C (PKC) activation. In PKC depleted J774 cells, PA induction could be elicited by chrysotile whereas, as expected, the response to phorbol myristate acetate (PMA) was abolished. The effect of PMA and chrysotile on the distribution of PKC activity in the J774 cell line was also compared by measuring the enzyme catalytic activity and phorbol dibutyrate (PDBu) binding sites. No redistribution of PKC was observed after stimulation with PA inducing doses of chrysotile, whereas a clear translocation was observed with PMA. It is concluded that the mechanism of PA induction by chrysotile in this macrophage-like cell line is independent of PKC activation.

References

Lison D, Knoops B, Lauwerys R . Effect of retinoic acid on asbestos induced plasminogen activator activity of peritoneal macrophages. Br J Ind Med. 1989; 46(7):496-7. PMC: 1009814. DOI: 10.1136/oem.46.7.496. View

Knoops B, Lison D, Collette C, Lauwerys R, Picard J . Plasminogen activator activity of normal and retinoic acid-treated post-implantation embryos. Biochem Pharmacol. 1990; 39(10):1545-8. DOI: 10.1016/0006-2952(90)90519-q. View

NISHIZUKA Y . The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature. 1988; 334(6184):661-5. DOI: 10.1038/334661a0. View

Robinson B . Production of plasminogen activator by alveolar macrophages in normal subjects and patients with interstitial lung disease. Thorax. 1988; 43(7):508-15. PMC: 461353. DOI: 10.1136/thx.43.7.508. View

Lison D, Raguzzi F, Lauwerys R . Comparison of the effects of auranofin, heavy metals and retinoids on protein kinase C in vitro and on a protein kinase C mediated response in macrophages. Pharmacol Toxicol. 1990; 67(3):239-42. DOI: 10.1111/j.1600-0773.1990.tb00820.x. View

Cantin A, Allard C, Begin R . Increased alveolar plasminogen activator in early asbestosis. Am Rev Respir Dis. 1989; 139(3):604-9. DOI: 10.1164/ajrccm/139.3.604. View

Roney P, Holian A . Possible mechanism of chrysotile asbestos-stimulated superoxide anion production in guinea pig alveolar macrophages. Toxicol Appl Pharmacol. 1989; 100(1):132-44. DOI: 10.1016/0041-008x(89)90097-5. View

Hamilton J, Vassalli J, Reich E . Macrophage plasminogen activator: induction by asbestos is blocked by anti-inflammatory steroids. J Exp Med. 1976; 144(6):1689-94. PMC: 2190481. DOI: 10.1084/jem.144.6.1689. View

Crooke S, SNYDER R, Butt T, Ecker D, Allaudeen H, Monia B . Cellular and molecular pharmacology of auranofin and related gold complexes. Biochem Pharmacol. 1986; 35(20):3423-31. DOI: 10.1016/0006-2952(86)90608-8. View

10.

Wallenstein S, Zucker C, Fleiss J . Some statistical methods useful in circulation research. Circ Res. 1980; 47(1):1-9. DOI: 10.1161/01.res.47.1.1. View

11.

Mossman B, Light W, Wei E . Asbestos: mechanisms of toxicity and carcinogenicity in the respiratory tract. Annu Rev Pharmacol Toxicol. 1983; 23:595-615. DOI: 10.1146/annurev.pa.23.040183.003115. View