Poly I:C-induced Expression of Intercellular Adhesion Molecule-1 in Intestinal Epithelial Cells

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 2009 Mar 17

PMID 19284409

Citations 11

Authors

D Omagari

Y Mikami

H Suguro

K Sunagawa

M Asano

E Sanuki

I Moro

K Komiyama

Affiliations

Soon will be listed here.

Abstract

Intercellular adhesion molecul-1 (ICAM-1) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily of adhesion molecules and plays perdominant roles in recruitment and trafficking of leucocytes to sites of inflammation. ICAM-1 expression in intestinal epithelial cells (IECs) is enhanced by several stimuli, such as proinflammatory cytokines, bacterial infections or pathogen-associated molecular patterns. One of these stimuli, double-stranded RNA (dsRNA), is a by-product of viral replication and can be recognized by its cognate receptor Toll-like receptor 3 (TLR-3). In spite of expression of both TLR-3 and ICAM-1 in IECs, correlation between TLR-3-signalling and ICAM-1 expression has never been examined in IECs. In the present study, we investigated whether poly I:C, an analogue of dsRNA, can stimulate the expression of ICAM-1 in IEC line, HT-29. Poly I:C-stimulation up-regulated the expression of ICAM-1 mRNA by real-time polymerase chain reaction. Enhanced expression of ICAM-1 was confirmed in protein level by immunofluoresense cell staining and enzyme-linked immunosorbent assay by measuring the released soluble ICAM-1 in culture supernatant. As the stimulation effect was reduced by pre-treatment of the cells with anti-TLR-3 antibody, poly I:C-binding signal was thought to be sensed by TLR-3 on the surface of HT-29. The results of luciferase assay and nuclear factor kappa-b (NF-kappaB) inhibitor treatment experiments indicated that the downstream signal was mainly transduced by transcription factor, NF-kappaB. All these results demonstrated the connection between TLR-3 signalling and ICAM-1 expression in HT-29 cells and indicated the importance of coordinated function of both innate and adaptive immunity against viral infections.

Citing Articles

Cell Surface Expression of Endosomal Toll-Like Receptors-A Necessity or a Superfluous Duplication?.

Mielcarska M, Bossowska-Nowicka M, Toka F Front Immunol. 2021; 11:620972.

PMID: 33597952 PMC: 7882679. DOI: 10.3389/fimmu.2020.620972.

Acid-electrolyzed functional water induces extracellular matrix metalloproteinase inducer, a possible novel alarmin, secretion from oral squamous cell carcinoma cell lines.

Kusunoki M, Sata E, Nishio K, Tanaka T, Nishida T, Sugano N Int J Med Sci. 2018; 15(12):1365-1372.

PMID: 30275765 PMC: 6158666. DOI: 10.7150/ijms.26186.

Nickel chloride administration prevents the growth of oral squamous cell carcinoma.

Ota H, Shionome T, Suguro H, Saito S, Ueki K, Arai Y Oncotarget. 2018; 9(35):24109-24121.

PMID: 29844876 PMC: 5963632. DOI: 10.18632/oncotarget.25313.

EMMPRIN Inhibits bFGF-Induced IL-6 Secretion in an Osteoblastic Cell Line, MC3T3-E1.

Saiki A, Motoyoshi M, Motozawa K, Okamura T, Ueki K, Shimizu N Int J Med Sci. 2017; 14(12):1173-1180.

PMID: 29104472 PMC: 5666549. DOI: 10.7150/ijms.20387.

Cancer cell-derived IL-8 induces monocytic THP1 cells to secrete IL-8 via the mitogen-activated protein kinase pathway.

Nishio Y, Gojoubori T, Kaneko Y, Shimizu N, Asano M Tumour Biol. 2015; 36(12):9171-7.

PMID: 26088451 DOI: 10.1007/s13277-015-3641-6.

References

Furrie E, Macfarlane S, Thomson G, Macfarlane G . Toll-like receptors-2, -3 and -4 expression patterns on human colon and their regulation by mucosal-associated bacteria. Immunology. 2005; 115(4):565-74. PMC: 1782176. DOI: 10.1111/j.1365-2567.2005.02200.x. View

Huang F, Kao C, Wachi S, Thai P, Ryu J, Wu R . Requirement for both JAK-mediated PI3K signaling and ACT1/TRAF6/TAK1-dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells. J Immunol. 2007; 179(10):6504-13. DOI: 10.4049/jimmunol.179.10.6504. View

Eckmann L, Kagnoff M . Intestinal mucosal responses to microbial infection. Springer Semin Immunopathol. 2005; 27(2):181-96. DOI: 10.1007/s00281-005-0207-5. View

Takeda K, Kaisho T, Akira S . Toll-like receptors. Annu Rev Immunol. 2003; 21:335-76. DOI: 10.1146/annurev.immunol.21.120601.141126. View

Bloom S, Simmons D, Jewell D . Adhesion molecules intercellular adhesion molecule-1 (ICAM-1), ICAM-3 and B7 are not expressed by epithelium in normal or inflamed colon. Clin Exp Immunol. 1995; 101(1):157-63. PMC: 1553302. DOI: 10.1111/j.1365-2249.1995.tb02292.x. View

Asano M, Ogura Y, Takenouchi-Ohkubo N, Chihaya H, Chung-Hsing W, Ishikawa K . Endoplasmic reticulum resident, immunoglobulin joining chain, can be secreted by perturbation of the calcium concentration in the endoplasmic reticulum. DNA Cell Biol. 2004; 23(7):403-11. DOI: 10.1089/1044549041474779. View

Schaefer T, Fahey J, Wright J, Wira C . Innate immunity in the human female reproductive tract: antiviral response of uterine epithelial cells to the TLR3 agonist poly(I:C). J Immunol. 2005; 174(2):992-1002. DOI: 10.4049/jimmunol.174.2.992. View

Akira S, Uematsu S, Takeuchi O . Pathogen recognition and innate immunity. Cell. 2006; 124(4):783-801. DOI: 10.1016/j.cell.2006.02.015. View

Staunton D, Merluzzi V, Rothlein R, Barton R, Marlin S, Springer T . A cell adhesion molecule, ICAM-1, is the major surface receptor for rhinoviruses. Cell. 1989; 56(5):849-53. DOI: 10.1016/0092-8674(89)90689-2. View

10.

Hopkins A, Baird A, Nusrat A . ICAM-1: targeted docking for exogenous as well as endogenous ligands. Adv Drug Deliv Rev. 2004; 56(6):763-78. DOI: 10.1016/j.addr.2003.10.043. View

11.

Brandtzaeg P . Molecular and cellular aspects of the secretory immunoglobulin system. APMIS. 1995; 103(1):1-19. DOI: 10.1111/j.1699-0463.1995.tb01073.x. View

12.

Doyle S, Vaidya S, OConnell R, Dadgostar H, Dempsey P, Wu T . IRF3 mediates a TLR3/TLR4-specific antiviral gene program. Immunity. 2002; 17(3):251-63. DOI: 10.1016/s1074-7613(02)00390-4. View

13.

Cario E, Podolsky D . Differential alteration in intestinal epithelial cell expression of toll-like receptor 3 (TLR3) and TLR4 in inflammatory bowel disease. Infect Immun. 2000; 68(12):7010-7. PMC: 97811. DOI: 10.1128/IAI.68.12.7010-7017.2000. View

14.

Matsumoto M, Kikkawa S, Kohase M, Miyake K, Seya T . Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun. 2002; 293(5):1364-9. DOI: 10.1016/S0006-291X(02)00380-7. View

15.

Jobin C, Hellerbrand C, Licato L, Brenner D, Sartor R . Mediation by NF-kappa B of cytokine induced expression of intercellular adhesion molecule 1 (ICAM-1) in an intestinal epithelial cell line, a process blocked by proteasome inhibitors. Gut. 1998; 42(6):779-87. PMC: 1727151. DOI: 10.1136/gut.42.6.779. View

16.

Matsumoto M, Funami K, Tanabe M, Oshiumi H, Shingai M, Seto Y . Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol. 2003; 171(6):3154-62. DOI: 10.4049/jimmunol.171.6.3154. View

17.

Yamamoto M, Sato S, Hemmi H, Hoshino K, Kaisho T, Sanjo H . Role of adaptor TRIF in the MyD88-independent toll-like receptor signaling pathway. Science. 2003; 301(5633):640-3. DOI: 10.1126/science.1087262. View

18.

Kelly C, OKeane J, Orellana J, Schroy 3rd P, Yang S, Lamont J . Human colon cancer cells express ICAM-1 in vivo and support LFA-1-dependent lymphocyte adhesion in vitro. Am J Physiol. 1992; 263(6 Pt 1):G864-70. DOI: 10.1152/ajpgi.1992.263.6.G864. View

19.

Huang G, Zhang X, Park N . Increased ICAM-1 expression in transformed human oral epithelial cells: molecular mechanism and functional role in peripheral blood mononuclear cell adhesion and lymphokine-activated-killer cell cytotoxicity. Int J Oncol. 2000; 17(3):479-86. PMC: 3513339. View

20.

Matsukura S, Kokubu F, Kurokawa M, Kawaguchi M, Ieki K, Kuga H . Synthetic double-stranded RNA induces multiple genes related to inflammation through Toll-like receptor 3 depending on NF-kappaB and/or IRF-3 in airway epithelial cells. Clin Exp Allergy. 2006; 36(8):1049-62. DOI: 10.1111/j.1365-2222.2006.02530.x. View