Increased ICAM-1 Expression in Transformed Human Oral Epithelial Cells: Molecular Mechanism and Functional Role in Peripheral Blood Mononuclear Cell Adhesion and Lymphokine-activated-killer Cell Cytotoxicity

Overview

Journal Int J Oncol

Specialty Oncology

Date 2000 Aug 12

PMID 10938387

Citations 7

Authors

G T Huang

X Zhang

N H Park

Affiliations

Soon will be listed here.

Abstract

The intercellular adhesion molecule-1 (ICAM-1, CD54) serves as a counter-receptor for the beta2-integrins, LFA-1 and Mac-1, which are expressed on leukocytes. Although expression of ICAM-1 on tumor cells has a role in tumor progression and development, information on ICAM-1 expression and its role in oral cancer has not been established. Normal human oral keratinocytes (NHOK), human papilloma virus (HPV)-immortalized human oral keratinocyte lines (HOK-16B, HOK-18A, and HOK-18C), and six human oral neoplastic cell lines (HOK-16B-BaP-T1, SCC-4, SCC-9, HEp-2, Tu-177 and 1483) were used to study ICAM-1 expression and its functional role in vitro. Our results demonstrated that NHOK express negligible levels of ICAM-1, whereas immortalized human oral keratinocytes and cancer cells express significantly higher levels of ICAM-1, except for HOK-16B-BaP-T1 and HEp-2. Altered mRNA half-lives did not fully account for the increased accumulation of ICAM-1 mRNA. Adhesion of peripheral blood mononuclear cells (PBMC) to epithelial cells correlated with cell surface ICAM-1 expression levels. This adhesion was inhibited by antibodies specific for either ICAM-1 or LFA-1/Mac-1, suggesting a role for these molecules in adhesion. In contrast, lymphokine-activated-killer (LAK) cell cytotoxic killing of epithelial cells did not correlate with ICAM-1 levels or with adhesion. Nonetheless, within each cell line, blocking of ICAM-1 or LFA-1/Mac-1 reduced LAK cell killing, suggesting that ICAM-1 is involved in mediating this killing.

Citing Articles

Behaviour of Human Oral Epithelial Cells Grown on Invisalign SmartTrack Material.

Nemec M, Bartholomaeus H, Bertl M, Behm C, Shokoohi-Tabrizi H, Jonke E Materials (Basel). 2020; 13(23).

PMID: 33255259 PMC: 7727678. DOI: 10.3390/ma13235311.

Enhancement of Programmed Death Ligand 2 on Hepatitis C Virus Infected Hepatocytes by Calcineurin Inhibitors.

Koike K, Takaki A, Yagi T, Iwasaki Y, Yasunaka T, Sadamori H Transplantation. 2015; 99(7):1447-54.

PMID: 25675203 PMC: 4539199. DOI: 10.1097/TP.0000000000000572.

Kaposi's sarcoma-associated herpesvirus ORF18 and ORF30 are essential for late gene expression during lytic replication.

Gong D, Wu N, Xie Y, Feng J, Tong L, Brulois K J Virol. 2014; 88(19):11369-82.

PMID: 25056896 PMC: 4178812. DOI: 10.1128/JVI.00793-14.

Poly I:C-induced expression of intercellular adhesion molecule-1 in intestinal epithelial cells.

Omagari D, Mikami Y, Suguro H, Sunagawa K, Asano M, Sanuki E Clin Exp Immunol. 2009; 156(2):294-302.

PMID: 19284409 PMC: 2759478. DOI: 10.1111/j.1365-2249.2009.03892.x.

Capacity of human beta-defensin expression in gene-transduced and cytokine-induced cells.

Yin C, Dang H, Zhang H, Gazor F, Kim D, Sorensen O Biochem Biophys Res Commun. 2005; 339(1):344-54.

PMID: 16298338 PMC: 3282591. DOI: 10.1016/j.bbrc.2005.11.020.

References

Makgoba M, Sanders M, Ginther Luce G, Gugel E, Dustin M, Springer T . Functional evidence that intercellular adhesion molecule-1 (ICAM-1) is a ligand for LFA-1-dependent adhesion in T cell-mediated cytotoxicity. Eur J Immunol. 1988; 18(4):637-40. DOI: 10.1002/eji.1830180423. View

Johnson J, Stade B, Holzmann B, Schwable W, Riethmuller G . De novo expression of intercellular-adhesion molecule 1 in melanoma correlates with increased risk of metastasis. Proc Natl Acad Sci U S A. 1989; 86(2):641-4. PMC: 286528. DOI: 10.1073/pnas.86.2.641. View

Griffiths C, Voorhees J, Nickoloff B . Characterization of intercellular adhesion molecule-1 and HLA-DR expression in normal and inflamed skin: modulation by recombinant gamma interferon and tumor necrosis factor. J Am Acad Dermatol. 1989; 20(4):617-29. DOI: 10.1016/s0190-9622(89)70073-6. View

Natali P, Nicotra M, Cavaliere R, Bigotti A, Romano G, Temponi M . Differential expression of intercellular adhesion molecule 1 in primary and metastatic melanoma lesions. Cancer Res. 1990; 50(4):1271-8. View

Diamond M, Staunton D, de Fougerolles A, Stacker S, Garcia-Aguilar J, Hibbs M . ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). J Cell Biol. 1990; 111(6 Pt 2):3129-39. PMC: 2116396. DOI: 10.1083/jcb.111.6.3129. View

Park N, Min B, Li S, Huang M, Cherick H, DONIGER J . Immortalization of normal human oral keratinocytes with type 16 human papillomavirus. Carcinogenesis. 1991; 12(9):1627-31. DOI: 10.1093/carcin/12.9.1627. View

Rosenstein Y, Park J, Hahn W, Rosen F, Bierer B, Burakoff S . CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1. Nature. 1991; 354(6350):233-5. DOI: 10.1038/354233a0. View

Akella R, Hall R . Expression of the adhesion molecules ICAM-1 and ICAM-2 on tumor cell lines does not correlate with their susceptibility to natural killer cell-mediated cytolysis: evidence for additional ligands for effector cell beta integrins. Eur J Immunol. 1992; 22(4):1069-74. DOI: 10.1002/eji.1830220429. View

Norris D . The state of differentiation of cultured human keratinocytes determines the level of intercellular adhesion molecule-1 (ICAM-1) expression induced by gamma interferon. J Invest Dermatol. 1992; 98(5):741-7. DOI: 10.1111/1523-1747.ep12499938. View

10.

Crawford J . Distribution of ICAM-1, LFA-3 and HLA-DR in healthy and diseased gingival tissues. J Periodontal Res. 1992; 27(4 Pt 1):291-8. DOI: 10.1111/j.1600-0765.1992.tb01680.x. View

11.

Coleman N, Greenfield I, Hare J, Chain B, Stanley M . Characterization and functional analysis of the expression of intercellular adhesion molecule-1 in human papillomavirus-related disease of cervical keratinocytes. Am J Pathol. 1993; 143(2):355-67. PMC: 1887013. View

12.

Kim M, Li S, Bertolami C, Cherrick H, Park N . State of p53, Rb and DCC tumor suppressor genes in human oral cancer cell lines. Anticancer Res. 1993; 13(5A):1405-13. View

13.

Katsanis E, Bausero M, Xu H, Orchard P, Xu Z, McIvor R . Transfection of the mouse ICAM-1 gene into murine neuroblastoma enhances susceptibility to lysis, reduces in vivo tumorigenicity and decreases ICAM-2-dependent killing. Cancer Immunol Immunother. 1994; 38(2):135-41. PMC: 11038452. DOI: 10.1007/BF01526209. View

14.

Gemmell E, Walsh L, Savage N, Seymour G . Adhesion molecule expression in chronic inflammatory periodontal disease tissue. J Periodontal Res. 1994; 29(1):46-53. DOI: 10.1111/j.1600-0765.1994.tb01090.x. View

15.

Shin K, Min B, Cherrick H, Park N . Combined effects of human papillomavirus-18 and N-methyl-N'-nitro-N-nitrosoguanidine on the transformation of normal human oral keratinocytes. Mol Carcinog. 1994; 9(2):76-86. DOI: 10.1002/mc.2940090205. View

16.

Vignola A, Chanez P, Campbell A, Pinel A, Bousquet J, Michel F . Quantification and localization of HLA-DR and intercellular adhesion molecule-1 (ICAM-1) molecules on bronchial epithelial cells of asthmatics using confocal microscopy. Clin Exp Immunol. 1994; 96(1):104-9. PMC: 1534530. DOI: 10.1111/j.1365-2249.1994.tb06238.x. View

17.

Chong A, Boussy I, Jiang X, Lamas M, Graf Jr L . CD54/ICAM-1 is a costimulator of NK cell-mediated cytotoxicity. Cell Immunol. 1994; 157(1):92-105. DOI: 10.1006/cimm.1994.1208. View

18.

Miele M, Bennett C, Miller B, Welch D . Enhanced metastatic ability of TNF-alpha-treated malignant melanoma cells is reduced by intercellular adhesion molecule-1 (ICAM-1, CD54) antisense oligonucleotides. Exp Cell Res. 1994; 214(1):231-41. DOI: 10.1006/excr.1994.1253. View

19.

Jahnke A, van de Stolpe A, Caldenhoven E, Johnson J . Constitutive expression of human intercellular adhesion molecule-1 (ICAM-1) is regulated by differentially active enhancing and silencing elements. Eur J Biochem. 1995; 228(2):439-46. View

20.

Ito M, Watanabe M, Ihara T, Kamiya H, Sakurai M . Increased expression of adhesion molecules (CD54, CD29 and CD44) on fibroblasts infected with cytomegalovirus. Microbiol Immunol. 1995; 39(2):129-33. DOI: 10.1111/j.1348-0421.1995.tb02179.x. View