SRBC/cavin-3 is a Caveolin Adapter Protein That Regulates Caveolae Function

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2009 Mar 6

PMID 19262564

Citations 104

Authors

Kerrie-Ann McMahon

Hubert Zajicek

Wei-Ping Li

Michael J Peyton

John D Minna

V James Hernandez

Katherine Luby-Phelps

Richard G W Anderson

Affiliations

Soon will be listed here.

Abstract

Caveolae are a major membrane domain common to most cells. One of the defining features of this domain is the protein caveolin. The exact function of caveolin, however, is not clear. One possible function is to attract adapter molecules to caveolae in a manner similar to how clathrin attracts molecules to coated pits. Here, we characterize a candidate adapter molecule called SRBC. SRBC binds PKCdelta and is a member of the STICK (substrates that interact with C-kinase) superfamily of PKC-binding proteins. We also show it co-immunoprecipitates with caveolin-1. A leucine zipper in SRBC is essential for both co-precipitation with caveolin and localization to caveolae. SRBC remains associated with caveolin when caveolae bud to form vesicles (cavicles) that travel on microtubules to different regions of the cell. In the absence of SRBC, intracellular cavicle traffic is markedly impaired. We conclude that SRBC (sdr-related gene product that binds to c-kinase) and two other family members [PTRF (Pol I and transcription release factor) and SDPR] function as caveolin adapter molecules that regulate caveolae function.

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