Functional Analysis of the Tobacco Mosaic Virus TRNA-like Structure in Cytoplasmic Gene Regulation

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1991 Sep 25

PMID 1923770

Citations 18

Authors

D R Gallie

J N Feder

R T Schimke

V Walbot

Affiliations

Soon will be listed here.

Abstract

The 3'-untranslated region (UTR) of tobacco mosaic virus (TMV), which terminates in a tRNA-like structure, functionally substitutes for a poly(A) tail in both plant and animal cells. The addition of the TMV 3'-UTR to chimeric mRNA constructs increases their expression up to 100-fold, increasing both translational efficiency and mRNA stability. The domain largely responsible for the regulation maps to a 72 base region immediately upstream of the tRNA-like structure, however, the 3'-terminal, tRNA-like structure is required for full function. Its contribution is lost if separated from the upstream pseudoknot domain by as few as 5 bases or if 6 bases are removed from the 3'-terminus. Sequence addition to the 3'-terminus of the TMV 3'UTR or the upstream pseudoknot domain inhibits function in both tobacco and Chinese hamster ovary cells.

Citing Articles

Non-canonical Translation in Plant RNA Viruses.

Miras M, Miller W, Truniger V, Aranda M Front Plant Sci. 2017; 8:494.

PMID: 28428795 PMC: 5382211. DOI: 10.3389/fpls.2017.00494.

TMV mutants with poly(A) tracts of different lengths demonstrate structural variations in 3'UTR affecting viral RNAs accumulation and symptom expression.

Guo S, Kierzek E, Chen G, Zhou Y, Wong S Sci Rep. 2015; 5:18412.

PMID: 26678425 PMC: 4683447. DOI: 10.1038/srep18412.

In vitro secondary structure of the genomic RNA of satellite tobacco mosaic virus.

Athavale S, Gossett J, Bowman J, Hud N, Williams L, Harvey S PLoS One. 2013; 8(1):e54384.

PMID: 23349871 PMC: 3551766. DOI: 10.1371/journal.pone.0054384.

Role of tRNA-like structures in controlling plant virus replication.

Dreher T Virus Res. 2008; 139(2):217-29.

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Step-wise formation of eukaryotic double-row polyribosomes and circular translation of polysomal mRNA.

Kopeina G, Afonina Z, Gromova K, Shirokov V, Vasiliev V, Spirin A Nucleic Acids Res. 2008; 36(8):2476-88.

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