Identification of a Chemical Probe for NAADP by Virtual Screening

Overview

Journal Nat Chem Biol

Specialties Biochemistry
Biology
Chemistry

Date 2009 Feb 24

PMID 19234453

Citations 171

Authors

Edmund Naylor

Abdelilah Arredouani

Sridhar R Vasudevan

Alexander M Lewis

Raman Parkesh

Akiko Mizote

Daniel Rosen

Justyn M Thomas

Minoru Izumi

A Ganesan

Antony Galione

Grant C Churchill

Affiliations

Soon will be listed here.

Abstract

Research into the biological role of the Ca(2+)-releasing second messenger NAADP (nicotinic acid adenine dinucleotide phosphate) has been hampered by a lack of chemical probes. To find new chemical probes for exploring NAADP signaling, we turned to virtual screening, which can evaluate millions of molecules rapidly and inexpensively. We used NAADP as the query ligand to screen the chemical library ZINC for compounds with similar three-dimensional shape and electrostatic properties. We tested the top-ranking hits in a sea urchin egg bioassay and found that one hit, Ned-19, blocks NAADP signaling at nanomolar concentrations. In intact cells, Ned-19 blocked NAADP signaling and fluorescently labeled NAADP receptors. Moreover, we show the utility of Ned-19 as a chemical probe by using it to demonstrate that NAADP is a key causal link between glucose sensing and Ca(2+) increases in mouse pancreatic beta cells.

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