Ontogeny and Phagocytic Function of Baboon Lung Dendritic Cells

Overview

Journal Immunol Cell Biol

Publisher Wiley

Specialties Allergy & Immunology
Cell Biology

Date 2009 Feb 19

PMID 19223853

Citations 2

Authors

Shanjana Awasthi

Roman Wolf

Gary White

Affiliations

Soon will be listed here.

Abstract

Dendritic cells (DCs) are the most potent antigen-presenting cells, but the ontogeny and functions of lung DCs are not known during prenatal period. Here, we isolated lung DC population from fetal (125-175 days of gestation age) and adult baboons. The cells were stained with fluorochrome-conjugated-HLA-DP, DQ, DR, CD1a, CD11c, CD14, CD40, CD80, CD86, CD209, CMKLR1, ILT7-specific antibodies, and staining was analyzed by flow cytometry. The phagocytic function was investigated by incubating the cells with fluorescent-labeled Escherichia coli bioparticles and analyzed by flow cytometry and fluorescence microscopy. The fetal baboon lung DCs expressed low levels of HLA-DP, DQ, DR, CD11c and CD86 as compared to adult baboon lung DCs and showed distinct DC morphology. The fetal lung DCs were also less capable of phagocytosing E. coli as compared to the adult lung DCs (P<0.05). In conclusion, the fetal lung DCs are not only phenotypically immature, but also less efficient in phagocytosing E. coli.

Citing Articles

Lung dendritic cell developmental programming, environmental stimuli, and asthma in early periods of life.

Awasthi S, Singh B, Welliver R, Dietert R J Allergy (Cairo). 2012; 2012:176468.

PMID: 23209481 PMC: 3503332. DOI: 10.1155/2012/176468.

Surfactant protein-A and toll-like receptor-4 modulate immune functions of preterm baboon lung dendritic cell precursor cells.

Awasthi S, Madhusoodhanan R, Wolf R Cell Immunol. 2011; 268(2):87-96.

PMID: 21439559 PMC: 3104394. DOI: 10.1016/j.cellimm.2011.02.009.

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