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Sequence Analysis of Nephropathogenic Infectious Bronchitis Virus Strains of the Massachusetts Genotype in Beijing

Overview
Journal Avian Pathol
Publisher Informa Healthcare
Date 2009 Feb 3
PMID 19184943
Citations 18
Authors
H Li
H Yang
Affiliations
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Abstract

The first 440 nucleotides of the S1 part of the spike protein (S) gene of three nephropathogenic infection bronchitis virus (IBV) strains (BJ1, BJ2 and BJ3), isolated from three rural chicken farms of Beijing with outbreaks of avian infectious bronchitis, were sequenced after reverse transcriptasenested polymerase chain reaction amplification. The sequence data were analyzed and compared with the standard IBV strains M41, Beaudette, vaccine strain H120 and Chinese vaccine strain D41. The three IBV isolates and strain M41 showed more than 99.1 and 97.8% identity in nucleotide sequence and deduced amino acid sequence, respectively, suggesting that the three nephropathogenic IBV strains belonged to the Massachusetts genotype of IBV.

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