Quantitative and Fingerprint Analyses of Chinese Sweet Tea Plant ( Rubus Suavissimus S. Lee)

Overview

Journal J Agric Food Chem

Specialties Chemistry
Nutritional Sciences

Date 2009 Jan 14

PMID 19138116

Citations 8

Authors

Guixin Chou

Shun-Jun Xu

Dong Liu

Gar Yee Koh

Jian Zhang

Zhijun Liu

Affiliations

Soon will be listed here.

Abstract

The quality of botanical food is increasingly assessed by the content of multiple bioactive compounds. This study reports, for the first time, an HPLC fingerprinting method for the quality evaluation of Rubus suavissimus leaves possessing multiple bioactivities. Five constituents, gallic acid, rutin, ellagic acid, rubusoside, and steviol monoside, were quantified and used in developing qualitative chromatographic fingerprints. The limits of detection and quantification ranged from 0.29 to 37.86 mug/mL. The relative standard deviations (RSDs) of intra- and interday precisions were no more than 3.14 and 3.01%, respectively. The average recoveries were between 93.1 and 97.5%. The developed method was validated in the analysis 14 leaf samples with satisfactory results. The contents of the five marker compounds accounted for an average of about 6% w/w with a variability of 16% among the 14 samples collected from a single site and year. Gallic acid was the least, whereas steviol monoside the most, variable compound among the 14 leaf samples. The characteristic compound rubusoside that is responsible for the sweet taste accounted for 5% of leaf weight. The validated method can now be used to quantitatively and qualitatively assess the quality of R. suavissimus leaves as traditional beverage or potential medicines.

Citing Articles

Assembly and comparative analysis of the complete mitogenome of var. , an exceptional berry plant possessing sweet leaves.

Shi Y, Chen Z, Jiang J, Wu W, Xin Y, Zeng W Front Plant Sci. 2025; 15:1504687.

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A Novel β-Glucosidase From 1433 for Efficient Rubusoside Production From Stevioside.

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Development of a Multifunction Set Yogurt Using S. Lee (Chinese Sweet Tea) Extract.

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Histochemical assays of secretory trichomes and the structure and content of mineral nutrients in Rubus idaeus L. leaves.

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Chemical Fingerprint and Multicomponent Quantitative Analysis for the Quality Evaluation of Cyclocarya paliurus Leaves by HPLC-Q-TOF-MS.

Cao Y, Fang S, Yin Z, Fu X, Shang X, Yang W Molecules. 2017; 22(11).

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