No Exit: Targeting the Budding Process to Inhibit Filovirus Replication

Overview

Journal Antiviral Res

Publisher Elsevier

Date 2008 Dec 31

PMID 19114059

Citations 42

Authors

Ronald N Harty

Affiliations

Soon will be listed here.

Abstract

The filoviruses, Ebola and Marburg, cause severe hemorrhagic fever in humans and nonhuman primates, with high mortality rates. Although the filovirus replication pathway is now understood in considerable detail, no antiviral drugs have yet been developed that directly inhibit steps in the replication cycle. One potential target is the filovirus VP40 matrix protein, the key viral protein that drives the budding process, in part by mediating specific virus-host interactions to facilitate the efficient release of virions from the infected cell. This review will summarize current knowledge of key structural and functional domains of VP40 believed to be necessary for efficient budding of virions and virus-like particles. A better understanding of the structure and function of these key regions of VP40 will be crucial, as they may represent novel and rational targets for inhibitors of filovirus egress.

Citing Articles

Research progress on the mechanism of exosome-mediated virus infection.

Zhang H, Liu X, Shi J, Su X, Xie J, Meng Q Front Cell Infect Microbiol. 2024; 14:1418168.

PMID: 38988816 PMC: 11233549. DOI: 10.3389/fcimb.2024.1418168.

Computational and experimental identification of keystone interactions in Ebola virus matrix protein VP40 dimer formation.

Narkhede Y, Saxena R, Sharma T, Conarty J, Ramirez V, Motsa B Protein Sci. 2024; 33(5):e4978.

PMID: 38591637 PMC: 11002992. DOI: 10.1002/pro.4978.

Role of phosphatidic acid lipids on plasma membrane association of the Ebola virus matrix protein VP40.

Cioffi M, Husby M, Gerstman B, Stahelin R, Chapagain P Biochim Biophys Acta Mol Cell Biol Lipids. 2024; 1869(3):159464.

PMID: 38360201 PMC: 11687635. DOI: 10.1016/j.bbalip.2024.159464.

Minor changes in electrostatics robustly increase VP40 membrane binding, assembly, and budding of Ebola virus matrix protein derived virus-like particles.

Motsa B, Sharma T, Chapagain P, Stahelin R bioRxiv. 2024; .

PMID: 38352396 PMC: 10862912. DOI: 10.1101/2024.01.30.578092.

Designing a novel multi‑epitope vaccine against Ebola virus using reverse vaccinology approach.

Alizadeh M, Amini-Khoei H, Tahmasebian S, Ghatrehsamani M, Samani K, Edalatpanah Y Sci Rep. 2022; 12(1):7757.

PMID: 35545650 PMC: 9094136. DOI: 10.1038/s41598-022-11851-z.

References

Hartlieb B, Weissenhorn W . Filovirus assembly and budding. Virology. 2005; 344(1):64-70. DOI: 10.1016/j.virol.2005.09.018. View

McCarthy S, Licata J, Harty R . A luciferase-based budding assay for Ebola virus. J Virol Methods. 2006; 137(1):115-9. DOI: 10.1016/j.jviromet.2006.06.007. View

Sudol M, Chen H, Bougeret C, Einbond A, Bork P . Characterization of a novel protein-binding module--the WW domain. FEBS Lett. 1995; 369(1):67-71. DOI: 10.1016/0014-5793(95)00550-s. View

Noda T, Sagara H, Suzuki E, Takada A, Kida H, Kawaoka Y . Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP. J Virol. 2002; 76(10):4855-65. PMC: 136157. DOI: 10.1128/jvi.76.10.4855-4865.2002. View

Krammer A, Kirchhoff P, Jiang X, Venkatachalam C, Waldman M . LigScore: a novel scoring function for predicting binding affinities. J Mol Graph Model. 2005; 23(5):395-407. DOI: 10.1016/j.jmgm.2004.11.007. View

Han Z, Harty R . Packaging of actin into Ebola virus VLPs. Virol J. 2005; 2:92. PMC: 1334228. DOI: 10.1186/1743-422X-2-92. View

Patnaik A, Wills J . In vivo interference of Rous sarcoma virus budding by cis expression of a WW domain. J Virol. 2002; 76(6):2789-95. PMC: 135952. DOI: 10.1128/jvi.76.6.2789-2795.2002. View

Ruthel G, Demmin G, Kallstrom G, Javid M, Badie S, Will A . Association of ebola virus matrix protein VP40 with microtubules. J Virol. 2005; 79(8):4709-19. PMC: 1069569. DOI: 10.1128/JVI.79.8.4709-4719.2005. View

Schmitt A, Lamb R . Escaping from the cell: assembly and budding of negative-strand RNA viruses. Curr Top Microbiol Immunol. 2004; 283:145-96. DOI: 10.1007/978-3-662-06099-5_5. View

10.

Gottlinger H, Dorfman T, Sodroski J, Haseltine W . Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle release. Proc Natl Acad Sci U S A. 1991; 88(8):3195-9. PMC: 51412. DOI: 10.1073/pnas.88.8.3195. View

11.

Kolesnikova L, Bugany H, Klenk H, Becker S . VP40, the matrix protein of Marburg virus, is associated with membranes of the late endosomal compartment. J Virol. 2002; 76(4):1825-38. PMC: 135914. DOI: 10.1128/jvi.76.4.1825-1838.2002. View

12.

Zhai Q, Fisher R, Chung H, Myszka D, Sundquist W, Hill C . Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV. Nat Struct Mol Biol. 2007; 15(1):43-9. DOI: 10.1038/nsmb1319. View

13.

Bausch D, Sprecher A, Jeffs B, Boumandouki P . Treatment of Marburg and Ebola hemorrhagic fevers: a strategy for testing new drugs and vaccines under outbreak conditions. Antiviral Res. 2008; 78(1):150-61. DOI: 10.1016/j.antiviral.2008.01.152. View

14.

Timmins J, Scianimanico S, Schoehn G, Weissenhorn W . Vesicular release of ebola virus matrix protein VP40. Virology. 2001; 283(1):1-6. DOI: 10.1006/viro.2001.0860. View

15.

Martin-Serrano J, Zang T, Bieniasz P . Role of ESCRT-I in retroviral budding. J Virol. 2003; 77(8):4794-804. PMC: 152150. DOI: 10.1128/jvi.77.8.4794-4804.2003. View

16.

Garrus J, von Schwedler U, Pornillos O, Morham S, Zavitz K, Wang H . Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell. 2001; 107(1):55-65. DOI: 10.1016/s0092-8674(01)00506-2. View

17.

Kolesnikova L, Ryabchikova E, Shestopalov A, Becker S . Basolateral budding of Marburg virus: VP40 retargets viral glycoprotein GP to the basolateral surface. J Infect Dis. 2007; 196 Suppl 2:S232-6. DOI: 10.1086/520584. View

18.

Kolesnikova L, Berghofer B, Bamberg S, Becker S . Multivesicular bodies as a platform for formation of the Marburg virus envelope. J Virol. 2004; 78(22):12277-87. PMC: 525088. DOI: 10.1128/JVI.78.22.12277-12287.2004. View

19.

Welsch S, Muller B, Krausslich H . More than one door - Budding of enveloped viruses through cellular membranes. FEBS Lett. 2007; 581(11):2089-97. PMC: 7126970. DOI: 10.1016/j.febslet.2007.03.060. View

20.

Harvey K, Kumar S . Nedd4-like proteins: an emerging family of ubiquitin-protein ligases implicated in diverse cellular functions. Trends Cell Biol. 1999; 9(5):166-9. DOI: 10.1016/s0962-8924(99)01541-x. View