Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-mediated Gene Transfer for Clinical Trials

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2008 Dec 27

PMID 19110618

Authors

Tulin Budak-Alpdogan

Isabelle Riviere

Affiliations

Soon will be listed here.

Abstract

This chapter provides information about the oncoretroviral transduction of human hematopoietic stem/ progenitor cells under clinically applicable conditions. We describe in detail a short -60 h transduction protocol which consistently yields transduction efficiencies in the range of 30-50% with five different oncoretroviral vectors. We discuss a number of parameters that affect transduction efficiency, including the oncoretroviral vector characteristics, the vector stock collection, the source of CD34+ cells and transduction conditions.

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