One-step Nucleic Acid Amplification-a Molecular Method for the Detection of Lymph Node Metastases in Breast Cancer Patients; Results of the German Study Group

Overview

Journal Virchows Arch

Specialties Cell Biology
Molecular Biology
Pathology

Date 2008 Dec 23

PMID 19101726

Citations 38

Authors

Christian Schem

Nicolai Maass

Dirk O Bauerschlag

Martin H Carstensen

Thomas Loning

Christian Roder

Olivera Batic

Walter Jonat

Katharina Tiemann

Affiliations

Soon will be listed here.

Abstract

Sentinel lymph node (SN) biopsy is part of the staging procedure in breast cancer patients. In this study, we compared an intraoperative tool named one-step nucleic acid amplification (OSNA) to our routine histological investigation. OSNA consists of a short homogenization step followed by amplification of cytokeratin (CK) 19 mRNA directly from the lysate. To evaluate the performance of OSNA in comparison to histology, analysis of 343 axillary lymph nodes (ALN) from 93 breast cancer patients was performed with both methods. Discordant samples were subjected to other methods. If these tests supported the OSNA results, these samples were excluded from the study. The concordance rate was 91.8%, sensitivity 98.1%, and specificity 90.8% before and 95.5%, 100%, and 95.6%, respectively, after discordant case investigation. Our results show that OSNA is an excellent method for the detection of metastases in lymph nodes and can be applied as an intraoperative diagnostic approach.

Citing Articles

The diagnostic performance of the one-step nucleic acid amplification assay for the detection of sentinel lymph node metastases in cytokeratin 19-positive breast cancer: a PRISMA-compliant meta-analysis.

Liu M, Wang W, Wang Y Front Med (Lausanne). 2024; 11:1391621.

PMID: 39314227 PMC: 11416989. DOI: 10.3389/fmed.2024.1391621.

Diagnostic value of one-step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19-positive tumors: evidence from bioinformatics and meta-analysis.

Li K, Meng M, Zhang W, Li J, Wang Y, Zhou C Front Oncol. 2024; 14:1370709.

PMID: 38651158 PMC: 11033366. DOI: 10.3389/fonc.2024.1370709.

OSNA Total Tumor Load for the Prediction of Axillary Involvement in Breast Cancer Patients: Should We use Different Thresholds According to the Intrinsic Molecular Subtype? MOTTO Study.

Bernet L, Hardisson D, Rodrigo M, Cordoba A, Sancho M, Peg V Clin Pathol. 2023; 16:2632010X231183693.

PMID: 37534372 PMC: 10392164. DOI: 10.1177/2632010X231183693.

Non-exposed endoscopic wall-inversion surgery with one-step nucleic acid amplification for early gastrointestinal tumors: Personal experience and literature review.

Crafa F, Vanella S, Morante A, Catalano O, Pomykala K, Baiamonte M World J Gastroenterol. 2023; 29(24):3883-3898.

PMID: 37426319 PMC: 10324533. DOI: 10.3748/wjg.v29.i24.3883.

Micrometastasis in lymph nodes of colorectal cancer.

Yamamoto H Ann Gastroenterol Surg. 2022; 6(4):466-473.

PMID: 35847437 PMC: 9271024. DOI: 10.1002/ags3.12576.

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