Identification of an Alternative Nitrogenase System in Rhodospirillum Rubrum

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Sep 1

PMID 1909322

Citations 34

Authors

L J Lehman

G P Roberts

Affiliations

Soon will be listed here.

Abstract

A second nitrogenase activity has been demonstrated in Rhodospirillum rubrum. This nitrogenase is expressed whenever a strain lacks an active Mo nitrogenase because of physiological or genetic inactivation. The alternative nitrogenase is able to support growth on N2 in the absence of fixed N. V does not stimulate, nor does Mo or W inhibit, growth or activity under the conditions tested. The proteins responsible for this activity were identified by electrophoretic and immunological properties. The synthesis of these proteins was repressed by NH4+. The alternative nitrogenase reductase is ADP ribosylated in response to darkness by the system that regulates the activity of the Mo nitrogenase. The genes for the alternative nitrogenase have been cloned, and the alternative nitrogenase reductase has been expressed in an in vitro transcription-translation system.

Citing Articles

An outer membrane porin-lipoprotein complex modulates elongasome movement to establish cell curvature in Rhodospirillum rubrum.

Pohl S, Giacomelli G, Meyer F, Kleeberg V, Cohen E, Biboy J Nat Commun. 2024; 15(1):7616.

PMID: 39223154 PMC: 11369160. DOI: 10.1038/s41467-024-51790-z.

Cultivation driven transcriptomic changes in the wild-type and mutant strains of .

Jureckova K, Nykrynova M, Slaninova E, Fleuriot-Blitman H, Amstutz V, Hermankova K Comput Struct Biotechnol J. 2024; 23:2681-2694.

PMID: 39035834 PMC: 11259993. DOI: 10.1016/j.csbj.2024.06.023.

A Red Fluorescent Protein Reporter System Developed for Measuring Gene Expression in Photosynthetic Bacteria under Anaerobic Conditions.

Jiang M, Zeng Y, Cui L, Wang M, Zheng Y Microorganisms. 2022; 10(2).

PMID: 35208656 PMC: 8880563. DOI: 10.3390/microorganisms10020201.

The Spectroscopy of Nitrogenases.

Van Stappen C, Decamps L, Cutsail 3rd G, Bjornsson R, Henthorn J, Birrell J Chem Rev. 2020; 120(12):5005-5081.

PMID: 32237739 PMC: 7318057. DOI: 10.1021/acs.chemrev.9b00650.

Reactivity, Mechanism, and Assembly of the Alternative Nitrogenases.

Jasniewski A, Lee C, Ribbe M, Hu Y Chem Rev. 2020; 120(12):5107-5157.

PMID: 32129988 PMC: 7491575. DOI: 10.1021/acs.chemrev.9b00704.

References

Joerger R, Bishop P . Bacterial alternative nitrogen fixation systems. Crit Rev Microbiol. 1988; 16(1):1-14. DOI: 10.3109/10408418809104465. View

Lowery R, Saari L, Ludden P . Reversible regulation of the nitrogenase iron protein from Rhodospirillum rubrum by ADP-ribosylation in vitro. J Bacteriol. 1986; 166(2):513-8. PMC: 214634. DOI: 10.1128/jb.166.2.513-518.1986. View

Fitzmaurice W, Saari L, Lowery R, Ludden P, Roberts G . Genes coding for the reversible ADP-ribosylation system of dinitrogenase reductase from Rhodospirillum rubrum. Mol Gen Genet. 1989; 218(2):340-7. DOI: 10.1007/BF00331287. View

Ludden P, Lehman L, Roberts G . Reversible ADP-ribosylation of dinitrogenase reductase in a nifD- mutant of Rhodospirillum rubrum. J Bacteriol. 1989; 171(9):5210-1. PMC: 210344. DOI: 10.1128/jb.171.9.5210-5211.1989. View

Bonam D, Lehman L, Roberts G, Ludden P . Regulation of carbon monoxide dehydrogenase and hydrogenase in Rhodospirillum rubrum: effects of CO and oxygen on synthesis and activity. J Bacteriol. 1989; 171(6):3102-7. PMC: 210021. DOI: 10.1128/jb.171.6.3102-3107.1989. View

Joerger R, Jacobson M, Premakumar R, Wolfinger E, Bishop P . Nucleotide sequence and mutational analysis of the structural genes (anfHDGK) for the second alternative nitrogenase from Azotobacter vinelandii. J Bacteriol. 1989; 171(2):1075-86. PMC: 209704. DOI: 10.1128/jb.171.2.1075-1086.1989. View

Pau R, Mitchenall L, Robson R . Genetic evidence for an Azotobacter vinelandii nitrogenase lacking molybdenum and vanadium. J Bacteriol. 1989; 171(1):124-9. PMC: 209564. DOI: 10.1128/jb.171.1.124-129.1989. View

Saari L, Triplett E, Ludden P . Purification and properties of the activating enzyme for iron protein of nitrogenase from the photosynthetic bacterium Rhodospirillum rubrum. J Biol Chem. 1984; 259(24):15502-8. View

Woehle D, Lueddecke B, Ludden P . ATP-dependent and NAD-dependent modification of glutamine synthetase from Rhodospirillum rubrum in vitro. J Biol Chem. 1990; 265(23):13741-9. View

10.

Joerger R, Loveless T, Pau R, Mitchenall L, Simon B, Bishop P . Nucleotide sequences and mutational analysis of the structural genes for nitrogenase 2 of Azotobacter vinelandii. J Bacteriol. 1990; 172(6):3400-8. PMC: 209151. DOI: 10.1128/jb.172.6.3400-3408.1990. View

11.

Chory J, Kaplan S . The in vitro transcription-translation of DNA and RNA templates by extracts of Rhodopseudomonas sphaeroides. Optimization and comparison of template specificity with Escherichia coli extracts and in vivo synthesis. J Biol Chem. 1982; 257(24):15110-21. View

12.

Feinberg A, Vogelstein B . "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984; 137(1):266-7. DOI: 10.1016/0003-2697(84)90381-6. View

13.

Kanemoto R, Ludden P . Effect of ammonia, darkness, and phenazine methosulfate on whole-cell nitrogenase activity and Fe protein modification in Rhodospirillum rubrum. J Bacteriol. 1984; 158(2):713-20. PMC: 215488. DOI: 10.1128/jb.158.2.713-720.1984. View

14.

Ludden P, Preston G, Dowling T . Comparison of active and inactive forms of iron protein from Rhodospirillum rubrum. Biochem J. 1982; 203(3):663-8. PMC: 1158281. DOI: 10.1042/bj2030663. View

15.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

16.

Roberts G, MacNeil T, MacNeil D, Brill W . Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae. J Bacteriol. 1978; 136(1):267-79. PMC: 218657. DOI: 10.1128/jb.136.1.267-279.1978. View

17.

Ludden P, BURRIS R . Activating factor for the iron protein of nitrogenase from Rhodospirillum rubrum. Science. 1976; 194(4263):424-6. DOI: 10.1126/science.824729. View

18.

Bishop P, Premakumar R, Dean D, Jacobson M, Chisnell J, Rizzo T . Nitrogen Fixation by Azotobacter vinelandii Strains Having Deletions in Structural Genes for Nitrogenase. Science. 1986; 232(4746):92-4. DOI: 10.1126/science.232.4746.92. View

19.

Lehman L, Fitzmaurice W, Roberts G . The cloning and functional characterization of the nifH gene of Rhodospirillum rubrum. Gene. 1990; 95(1):143-7. DOI: 10.1016/0378-1119(90)90426-r. View

20.

POPE M, Murrell S, Ludden P . Covalent modification of the iron protein of nitrogenase from Rhodospirillum rubrum by adenosine diphosphoribosylation of a specific arginine residue. Proc Natl Acad Sci U S A. 1985; 82(10):3173-7. PMC: 397737. DOI: 10.1073/pnas.82.10.3173. View