Effect of Ammonia, Darkness, and Phenazine Methosulfate on Whole-cell Nitrogenase Activity and Fe Protein Modification in Rhodospirillum Rubrum

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1984 May 1

PMID 6427184

Citations 73

Authors

R H Kanemoto

P W Ludden

Affiliations

Soon will be listed here.

Abstract

A procedure for the immunoprecipitation of Fe protein from cell extracts was developed and used to monitor the modification of Fe protein in vivo. The subunit pattern of the isolated Fe protein after sodium dodecyl sulfate-polyacrylamide gel electrophoresis was assayed by Coomassie brilliant blue protein staining and autoradiographic 32P detection of the modifying group. Whole-cell nitrogenase activity was also monitored during Fe protein modification. The addition of ammonia, darkness, oxygen, carbonyl cyanide m-chlorophenylhydrazone, and phenazine methosulfate each resulted in a loss of whole-cell nitrogenase activity and the in vivo modification of Fe protein. For ammonia and darkness, the rate of loss of nitrogenase activity was similar to that for Fe protein modification. The reillumination of a culture incubated in the dark brought about a rapid recovery of nitrogenase activity and the demodification of Fe protein. Cyclic dark-light treatments resulted in matching cycles of nitrogenase activity and Fe protein modification. Carbonyl cyanide m-chlorophenylhydrazone and phenazine methosulfate treatments caused an immediate loss of nitrogenase activity, whereas Fe protein modification occurred at a slower rate. Oxygen treatment resulted in a rapid loss of activity but only an incomplete modification of the Fe protein.

Citing Articles

Potential of Phototrophic Purple Nonsulfur Bacteria to Fix Nitrogen in Rice Fields.

Maeda I Microorganisms. 2022; 10(1).

PMID: 35056477 PMC: 8777916. DOI: 10.3390/microorganisms10010028.

New Approach for the Construction and Calibration of Gas-Tight Setups for Biohydrogen Production at the Small Laboratory Scale.

Autenrieth C, Shaw S, Ghosh R Metabolites. 2021; 11(10).

PMID: 34677382 PMC: 8541310. DOI: 10.3390/metabo11100667.

Posttranslational modification of dinitrogenase reductase in Rhodospirillum rubrum treated with fluoroacetate.

Akentieva N World J Microbiol Biotechnol. 2018; 34(12):184.

PMID: 30488133 DOI: 10.1007/s11274-018-2564-y.

Diazotrophic bacterial community variability in a subtropical deep reservoir is correlated with seasonal changes in nitrogen.

Wang L, Yu Z, Yang J, Zhou J Environ Sci Pollut Res Int. 2015; 22(24):19695-705.

PMID: 26278898 DOI: 10.1007/s11356-015-5144-9.

Quorum sensing influences growth and photosynthetic membrane production in high-cell-density cultivations of Rhodospirillum rubrum.

Carius L, Carius A, McIntosh M, Grammel H BMC Microbiol. 2013; 13:189.

PMID: 23927486 PMC: 3751510. DOI: 10.1186/1471-2180-13-189.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Bonner W, Laskey R . A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974; 46(1):83-8. DOI: 10.1111/j.1432-1033.1974.tb03599.x. View

Haaker H, Laane C, Hellingwerf K, Houwer B, Konings W, Veeger C . Short-term regulation of the nitrogenase activity in Rhodopseudomonas sphaeroides. Eur J Biochem. 1982; 127(3):639-45. DOI: 10.1111/j.1432-1033.1982.tb06920.x. View

Jones B, MONTY K . Glutamine as a feedback inhibitor of the Rhodopseudomonas sphaeroides nitrogenase system. J Bacteriol. 1979; 139(3):1007-13. PMC: 218049. DOI: 10.1128/jb.139.3.1007-1013.1979. View

Schick H . Substrate and light dependent fixation of molecular nitrogen in Rhodospirillum rubrum. Arch Mikrobiol. 1971; 75(2):89-101. DOI: 10.1007/BF00407997. View

Hallenbeck P, Meyer C, Vignais P . Nitrogenase from the photosynthetic bacterium Rhodopseudomonas capsulata: purification and molecular properties. J Bacteriol. 1982; 149(2):708-17. PMC: 216563. DOI: 10.1128/jb.149.2.708-717.1982. View

NORDLUND S, Eriksson U, Baltscheffsky H . Necessity of a membrane component for nitrogenase activity in Rhodospirillum rubrum. Biochim Biophys Acta. 1977; 462(1):187-95. DOI: 10.1016/0005-2728(77)90201-8. View

Ormerod J, ORMEROD K, Gest H . Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys. 1961; 94:449-63. DOI: 10.1016/0003-9861(61)90073-x. View

Yoch D, Gotto J . Effect of light intensity and inhibitors of nitrogen assimilation on NH4+ inhibition of nitrogenase activity in Rhodospirillum rubrum and Anabaena sp. J Bacteriol. 1982; 151(2):800-6. PMC: 220328. DOI: 10.1128/jb.151.2.800-806.1982. View

10.

Pratt D, Frenkel A . Studies on Nitrogen Fixation and Photosynthesis of Rhodospirillum Rubrum. Plant Physiol. 1959; 34(3):333-7. PMC: 541200. DOI: 10.1104/pp.34.3.333. View

11.

Sweet W, BURRIS R . Inhibition of nitrogenase activity by NH+4 in Rhodospirillum rubrum. J Bacteriol. 1981; 145(2):824-31. PMC: 217185. DOI: 10.1128/jb.145.2.824-831.1981. View

12.

Johansson B, Gest H . Adenylylation/deadenylylation control of the glutamine synthetase of Rhodopseudomonas capsulata. Eur J Biochem. 1977; 81(2):365-71. DOI: 10.1111/j.1432-1033.1977.tb11960.x. View

13.

Bognar A, Desrosiers L, Libman M, Newman E . Control of nitrogenase in a photosynthetic autotrophic bacterium, Ectothiorhodospira sp. J Bacteriol. 1982; 152(2):706-13. PMC: 221520. DOI: 10.1128/jb.152.2.706-713.1982. View

14.

Dowling T, Preston G, Ludden P . Heat activation of the Fe protein of nitrogenase from Rhodospirillum rubrum. J Biol Chem. 1982; 257(23):13987-92. View

15.

MUNSON T, BURRIS R . Nitrogen fixation by Rhodospirillum rubrum grown in nitrogen-limited continuous culture. J Bacteriol. 1969; 97(3):1093-8. PMC: 249819. DOI: 10.1128/jb.97.3.1093-1098.1969. View

16.

Howard K, Hales B, Socolofsky M . Nitrogen fixation and ammonia switch-off in the photosynthetic bacterium Rhodopseudomonas viridis. J Bacteriol. 1983; 155(1):107-12. PMC: 217658. DOI: 10.1128/jb.155.1.107-112.1983. View

17.

Preston G, Ludden P . Change in subunit composition of the iron protein of nitrogenase from Rhodospirillum rubrum during activation and inactivation of iron protein. Biochem J. 1982; 205(3):489-94. PMC: 1158512. DOI: 10.1042/bj2050489. View

18.

Carithers R, Yoch D, Arnon D . Two forms of nitrogenase from the photosynthetic bacterium Rhodospirillum rubrum. J Bacteriol. 1979; 137(2):779-89. PMC: 218357. DOI: 10.1128/jb.137.2.779-789.1979. View

19.

Ludden P, BURRIS R . Activating factor for the iron protein of nitrogenase from Rhodospirillum rubrum. Science. 1976; 194(4263):424-6. DOI: 10.1126/science.824729. View

20.

Neilson A, NORDLUND S . Regulation of nitrogenase synthesis in intact cells of Rhodospirillum rubrum: inactivation of nitrogen fixation by ammonia, L-glutamine and L-asparagine. J Gen Microbiol. 1975; 91(1):53-62. DOI: 10.1099/00221287-91-1-53. View