A Newly Identified Bocavirus Species in Human Stool

Overview

Journal J Infect Dis

Publisher Oxford University Press

Specialty Infectious Diseases

Date 2008 Dec 17

PMID 19072716

Citations 164

Authors

Amit Kapoor

Elizabeth Slikas

Peter Simmonds

Thaweesak Chieochansin

Asif Naeem

Shahzad Shaukat

Muhammad Masroor Alam

Salmaan Sharif

Mehar Angez

Sohail Zaidi

Eric Delwart

Affiliations

Soon will be listed here.

Abstract

Viral metagenomic analysis was used to identify a previously uncharacterized parvovirus species, "HBoV2," whose closest phylogenetic relative is the human bocavirus (HBoV). HBoV2 has a genomic organization identical to that of HBoV but has only 78%, 67%, and 80% identity, respectively, with the latter's NS1, NP1, and VP1/VP2 proteins. The study used polymerase chain reaction to detect HBoV2 sequences in 5 of 98 stool samples from Pakistani children and in 3 of 699 stool samples from Edinburgh. Nearly-full-length genome sequencing revealed the presence of 3 HBoV2 genotypes and evidence of recombination between genotypes. Further studies are necessary to identify anatomical sites of HBoV2 replication and potential associations with clinical symptoms or disease.

Citing Articles

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References

Hokynar K, Soderlund-Venermo M, Pesonen M, Ranki A, Kiviluoto O, Partio E . A new parvovirus genotype persistent in human skin. Virology. 2002; 302(2):224-8. DOI: 10.1006/viro.2002.1673. View

Allander T, Tammi M, Eriksson M, Bjerkner A, Tiveljung-Lindell A, Andersson B . Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A. 2005; 102(36):12891-6. PMC: 1200281. DOI: 10.1073/pnas.0504666102. View

Norja P, Eis-Hubinger A, Soderlund-Venermo M, Hedman K, Simmonds P . Rapid sequence change and geographical spread of human parvovirus B19: comparison of B19 virus evolution in acute and persistent infections. J Virol. 2008; 82(13):6427-33. PMC: 2447064. DOI: 10.1128/JVI.00471-08. View

Kleinman S, Glynn S, Lee T, Tobler L, Montalvo L, Todd D . Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay. Transfusion. 2007; 47(10):1756-64. DOI: 10.1111/j.1537-2995.2007.01341.x. View

Manning A, Willey S, Bell J, Simmonds P . Comparison of tissue distribution, persistence, and molecular epidemiology of parvovirus B19 and novel human parvoviruses PARV4 and human bocavirus. J Infect Dis. 2007; 195(9):1345-52. PMC: 7109978. DOI: 10.1086/513280. View

Fryer J, Delwart E, Hecht F, Bernardin F, Jones M, Shah N . Frequent detection of the parvoviruses, PARV4 and PARV5, in plasma from blood donors and symptomatic individuals. Transfusion. 2007; 47(6):1054-61. DOI: 10.1111/j.1537-2995.2007.01235.x. View

Neske F, Blessing K, Tollmann F, Schubert J, Rethwilm A, Kreth H . Real-time PCR for diagnosis of human bocavirus infections and phylogenetic analysis. J Clin Microbiol. 2007; 45(7):2116-22. PMC: 1932993. DOI: 10.1128/JCM.00027-07. View

Cohen B, Gandhi J, Clewley J . Genetic variants of parvovirus B19 identified in the United Kingdom: implications for diagnostic testing. J Clin Virol. 2006; 36(2):152-5. DOI: 10.1016/j.jcv.2006.01.011. View

Shackelton L, Hoelzer K, Parrish C, Holmes E . Comparative analysis reveals frequent recombination in the parvoviruses. J Gen Virol. 2007; 88(Pt 12):3294-3301. PMC: 3326350. DOI: 10.1099/vir.0.83255-0. View

10.

Allander T . Human bocavirus. J Clin Virol. 2007; 41(1):29-33. DOI: 10.1016/j.jcv.2007.10.026. View

11.

Kantola K, Hedman L, Allander T, Jartti T, Lehtinen P, Ruuskanen O . Serodiagnosis of human bocavirus infection. Clin Infect Dis. 2008; 46(4):540-6. PMC: 7107971. DOI: 10.1086/526532. View

12.

Cossart Y, Field A, Cant B, Widdows D . Parvovirus-like particles in human sera. Lancet. 1975; 1(7898):72-3. DOI: 10.1016/s0140-6736(75)91074-0. View

13.

Jones M, Kapoor A, Lukashov V, Simmonds P, Hecht F, Delwart E . New DNA viruses identified in patients with acute viral infection syndrome. J Virol. 2005; 79(13):8230-6. PMC: 1143717. DOI: 10.1128/JVI.79.13.8230-8236.2005. View

14.

Lau S, Yip C, Que T, Lee R, Au-Yeung R, Zhou B . Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong. J Infect Dis. 2007; 196(7):986-93. PMC: 7111856. DOI: 10.1086/521310. View

15.

Horowitz B, Busch M . Estimating the pathogen safety of manufactured human plasma products: application to fibrin sealants and to thrombin. Transfusion. 2008; 48(8):1739-53. PMC: 7201864. DOI: 10.1111/j.1537-2995.2008.01717.x. View

16.

Chen K, Shull B, Moses E, Lederman M, Stout E, Bates R . Complete nucleotide sequence and genome organization of bovine parvovirus. J Virol. 1986; 60(3):1085-97. PMC: 253350. DOI: 10.1128/JVI.60.3.1085-1097.1986. View

17.

Kapoor A, Victoria J, Simmonds P, Wang C, Shafer R, Nims R . A highly divergent picornavirus in a marine mammal. J Virol. 2007; 82(1):311-20. PMC: 2224395. DOI: 10.1128/JVI.01240-07. View

18.

Norja P, Hokynar K, Aaltonen L, Chen R, Ranki A, Partio E . Bioportfolio: lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue. Proc Natl Acad Sci U S A. 2006; 103(19):7450-3. PMC: 1464359. DOI: 10.1073/pnas.0602259103. View

19.

Sloots T, Whiley D, Lambert S, Nissen M . Emerging respiratory agents: new viruses for old diseases?. J Clin Virol. 2008; 42(3):233-43. PMC: 7108325. DOI: 10.1016/j.jcv.2008.03.002. View

20.

Simmonds P, Douglas J, Bestetti G, Longhi E, Antinori S, Parravicini C . A third genotype of the human parvovirus PARV4 in sub-Saharan Africa. J Gen Virol. 2008; 89(Pt 9):2299-2302. DOI: 10.1099/vir.0.2008/001180-0. View