Simple Method to Accurately Differentiate Candida Albicans Isolates Concurrently Using Polymorphic Patterns of PCR-amplified, Species-specific Nuclear and Mitochondrial Targets

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2008 Nov 20

PMID 19018592

Authors

Yozo Miyakawa

Takuya Ozawa

Affiliations

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Abstract

We describe a simple method to accurately differentiate Candida albicans isolates by concurrent use of the restriction enzyme digestion patterns for PCR products, targeting two species-specific DNA regions originating from genetically different sources, the nuclear and mitochondrial genomes. The target sequence we used as the nuclear gene was derived from the PHO85 gene, a negative regulator of the PHO system, in which we found a restriction size polymorphism within the two alleles of PHO85 in the diploid genome of this fungus. The mitochondrial target was derived from EO3, a species-specific DNA fragment possessing a small size polymorphism among various clinical isolates. Our results should provide a new tool for molecular epidemiological surveys of patients suffering from candidiasis caused by C. albicans.

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