Lipid Modification of Proteins Through Sortase-catalyzed Transpeptidation

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2008 Nov 8

PMID 18989959

Citations 54

Authors

John M Antos

Gwenn M Miller

Gijsbert M Grotenbreg

Hidde L Ploegh

Affiliations

Soon will be listed here.

Abstract

A general chemoenzymatic method for the site-specific attachment of lipids to protein substrates is described. Sortase A is used to append short lipid-modified oligoglycine peptides to the C terminus of protein substrates bearing a five amino acid sortase A recognition sequence (LPETG). We demonstrate the attachment of a range of hydrophobic modifications in excellent yield (60-90%), including a simple step for removing the sortase enzyme postreaction. Lipoproteins prepared using these procedures were subsequently shown to associate with mammalian cells in a lipid tail-dependent fashion and localized to the plasma membrane and endosomes.

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The Transpeptidase Sortase A Binds Nucleic Acids and Mediates Mammalian Cell Labeling.

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