Infection Initiated by the RNA Pregenome of a DNA Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1991 Oct 1

PMID 1895392

Citations 16

Authors

M J Huang

J Summers

Affiliations

Soon will be listed here.

Abstract

We describe experiments demonstrating that after transfection into permissive cells, the RNA pregenome of an avian hepadnavirus, the duck hepatitis B virus, is infectious. Using a Sindbis virus expression vector, we showed that cytoplasmic synthesis of the pregenome resulted in hepadnaviral DNA synthesis. Moreover, complete infectious virus was produced from cells transfected with hepadnaviral pregenomic RNA. We conclude that the pregenome of hepadnaviruses can express all the proteins required for DNA synthesis as well as serve as a template for reverse transcription and that DNA resulting from pregenome expression can be utilized to establish a productive infection in pregenome-transfected cells.

Citing Articles

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VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells.

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Single-cell analysis of covalently closed circular DNA copy numbers in a hepadnavirus-infected liver.

Zhang Y, Zhang B, Theele D, Litwin S, Toll E, Summers J Proc Natl Acad Sci U S A. 2003; 100(21):12372-7.

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