Variations in Integration Site of Avian Oncornaviruses in Different Hosts

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1977 Feb 1

PMID 189085

Citations 1

Authors

M N Dastoor

M Shoyab

M A Baluda

Affiliations

Soon will be listed here.

Abstract

We examined the integration site of avian oncornaviruses in the genome of different hosts with respect to the repetitive frequency of the cellular DNA sequences adjacent to the integrated proviral DNA. The following systems were studied: avian sarcoma virus (B-77) and avian leukosis virus (Rous-associated virus-61) in cultured duck embryonic cells and B-77 in cultured mouse 3T3 cells. These systems represent different host responses to viral infection, i.e., one in which both cellular transformation and viral replication occur (B-77-infected duck cells), one in which viral replication, but not transformation, occurs (Rous-associated virus-61-infected duck cells), and one in which transformation, but not viral replication, occurs (B-77-infected 3T3 cells). Two sequential hybridizations were used. First, large denatured DNA fragments (2.8 X 10(6) daltons) were reassociated to different C0t (mole-seconds per liter) values. Next, DNA remaining single stranded at different C0t values was isolated by hydroxylapatite column chromatography, immobilized on nitrocellulose filters, and hybridized with an excess of 3H-labeled 35S viral RNA to titrate the concentration of proviral DNA. Results show that B-77 sarcoma virus and Rous-associated virus-61 integrate in the unique region of duck DNA, whereas B-77 proviral DNA is associated with both repeated and unique host DNA sequences in transformed mouse 3T3 cells.

Citing Articles

Sites of integration of reticuloendotheliosis virus DNA in chicken DNA.

Keshet E, Temin H Proc Natl Acad Sci U S A. 1978; 75(7):3372-6.

PMID: 210459 PMC: 392778. DOI: 10.1073/pnas.75.7.3372.

References

Varmus H, Shank P . Unintegrated viral DNA is synthesized in the cytoplasm of avian sarcoma virus-transformed duck cells by viral DNA polymerase. J Virol. 1976; 18(2):567-73. PMC: 515583. DOI: 10.1128/JVI.18.2.567-573.1976. View

Shoyab M, Baluda M, Evans R . Acquisition of new DNA sequences after infection of chicken cells with avian myeloblastosis virus. J Virol. 1974; 13(2):331-9. PMC: 355301. DOI: 10.1128/JVI.13.2.331-339.1974. View

Evans R, Shoyab M, Baluda M . Studies on characterization of the integration sites of avian RNA tumor virus-specific DNA. Cold Spring Harb Symp Quant Biol. 1975; 39 Pt 2:1005-7. DOI: 10.1101/sqb.1974.039.01.115. View

Martin R, Chou J, Avila J, Saral R . The semiautonomous replicon: a molecular model for the oncogenicity of SV40. Cold Spring Harb Symp Quant Biol. 1975; 39 Pt 1:17-24. DOI: 10.1101/sqb.1974.039.01.005. View

Shoyab M, Dastoor M, Baluda M . Evidence for tandem integration of avian myeloblastosis virus DNA with endogenous provirus in leukemic chicken cells. Proc Natl Acad Sci U S A. 1976; 73(5):1749-53. PMC: 430378. DOI: 10.1073/pnas.73.5.1749. View

Manning J, Schmid C, Davidson N . Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome. Cell. 1975; 4(2):141-55. DOI: 10.1016/0092-8674(75)90121-x. View

Pays E, Ronsse A . Interspersion of repetitive sequences in rat liver DNA. Biochem Biophys Res Commun. 1975; 62(4):862-7. DOI: 10.1016/0006-291x(75)90402-7. View

Davidson E, HOUGH B, Klein W, Britten R . Structural genes adjacent to interspersed repetitive DNA sequences. Cell. 1975; 4(3):217-38. DOI: 10.1016/0092-8674(75)90170-1. View

ROBINSON W, Baluda M . The nucleic acid from avian myeloblastosis virus compared with the RNA from the Bryan strain of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1965; 54(6):1686-92. PMC: 300535. DOI: 10.1073/pnas.54.6.1686. View

10.

Hirt B . Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967; 26(2):365-9. DOI: 10.1016/0022-2836(67)90307-5. View

11.

Sambrook J, Westphal H, Srinivasan P, DULBECCO R . The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968; 60(4):1288-95. PMC: 224916. DOI: 10.1073/pnas.60.4.1288. View

12.

Baluda M, Nayak D . Incorporation of precursors into ribonucleic acid, protein, glycoprotein, and lipoprotein of avian myeloblastosis virions. J Virol. 1969; 4(5):554-66. PMC: 375909. DOI: 10.1128/JVI.4.5.554-566.1969. View

13.

Baluda M . Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses. Proc Natl Acad Sci U S A. 1972; 69(3):576-80. PMC: 426510. DOI: 10.1073/pnas.69.3.576. View

14.

Temin H . The RNA tumor viruses--background and foreground. Proc Natl Acad Sci U S A. 1972; 69(4):1016-20. PMC: 426618. DOI: 10.1073/pnas.69.4.1016. View

15.

Markham P, Baluda M . Integrated state of oncornavirus DNA in normal chicken cells and in cells transformed by avian myeloblastosis virus. J Virol. 1973; 12(4):721-32. PMC: 356690. DOI: 10.1128/JVI.12.4.721-732.1973. View

16.

Varmus H, Vogt P, Bishop J . Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts. Proc Natl Acad Sci U S A. 1973; 70(11):3067-71. PMC: 427171. DOI: 10.1073/pnas.70.11.3067. View

17.

Ali M, Baluda M . Synthesis of avian oncornavirus DNA in infected chicken cells. J Virol. 1974; 13(5):1005-13. PMC: 355408. DOI: 10.1128/JVI.13.5.1005-1013.1974. View

18.

Shoyab M, Evans R, Baluda M . Presence in leukemic cells of avian myeloblastosis virus-specific DNA sequences absent in normal chicken cells. J Virol. 1974; 14(1):47-9. PMC: 355476. DOI: 10.1128/JVI.14.1.47-49.1974. View

19.

Evans R, Baluda M, Shoyab M . Differences between the integration of avian myeloblastosis virus DNA in leukemic cells and of endogenous viral DNA in normal chicken cells. Proc Natl Acad Sci U S A. 1974; 71(8):3152-6. PMC: 388640. DOI: 10.1073/pnas.71.8.3152. View

20.

Faust C, DIGGELMANN H, MACH B . Estimation of the number of genes coding for the constant part of the mouse immunoglobulin kappa light chain. Proc Natl Acad Sci U S A. 1974; 71(6):2491-5. PMC: 388485. DOI: 10.1073/pnas.71.6.2491. View