Evidence for Tandem Integration of Avian Myeloblastosis Virus DNA with Endogenous Provirus in Leukemic Chicken Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1976 May 1

PMID 179099

Citations 8

Authors

M Shoyab

M N Dastoor

M A Baluda

Affiliations

Soon will be listed here.

Abstract

The integration site of avian myeloblastosis virus (AMV) proviral DNA in DNA from leukemia chicken myeloblasts has been studied by three sequential nucleic acid hybridizations that can localize the proviral DNA according to the repetitiveness of the adjacent cellular DNA regions. First, large denatured cellular DNA fragments (2.1 x 10(6) daltons) were reassociated and fractionated according to sequence reiteration frequenct. Next, DNA remaining single-stranded in each fraction was immobilized on nitrocellulose filters hybridized with an excess of unlabeled 70S RNA from Rous-associated virus-0 to saturate the endogenous proviral DNA sequences.

Citing Articles

Integration of different sarcoma virus genomes into host DNA: evidence against tandem arrangement and for shared integration sites.

Akiyama Y, Vogt P Proc Natl Acad Sci U S A. 1979; 76(5):2465-9.

PMID: 221927 PMC: 383623. DOI: 10.1073/pnas.76.5.2465.

Measurement of proviral genes in uninfected and avian myeloblastosis virus-infected cells by hybridization with 3H-labeled complementary DNA probe excess.

Heilmann L, Herman T, Beaudreau G J Virol. 1977; 24(2):498-504.

PMID: 199736 PMC: 515959. DOI: 10.1128/JVI.24.2.498-504.1977.

Genetic variation in the RNA transcripts of endogenous virus genes in uninfected chicken cells.

Wang S, Hayward W, Hanafusa H J Virol. 1977; 24(1):64-73.

PMID: 198586 PMC: 515910. DOI: 10.1128/JVI.24.1.64-73.1977.

Integration of proviral DNA in chicken cells infected with Schmidt-Ruppin Rous sarcoma virus is not enhanced by DNA repair.

Tsuruo T, Baluda M J Virol. 1977; 23(3):533-42.

PMID: 197260 PMC: 515864. DOI: 10.1128/JVI.23.3.533-542.1977.

Homogeneity and complexity of avian oncornavirus proviral DNA determined by molecular hybridization.

Evans R, Shoyab M, Drohan W, Baluda M J Virol. 1977; 21(3):942-9.

PMID: 191654 PMC: 515632. DOI: 10.1128/JVI.21.3.942-949.1977.

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