Mitochondrial Inner Membrane Protease 1 of Saccharomyces Cerevisiae Shows Sequence Similarity to the Escherichia Coli Leader Peptidase

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1991 Aug 1

PMID 1886606

Citations 32

Authors

M Behrens

G Michaelis

E Pratje

Affiliations

Soon will be listed here.

Abstract

The nuclear yeast mutant pet ts2858 is defective in the removal of pre-sequences from the mitochondrially encoded cytochrome oxidase subunit II (COXII) and the processing intermediate of cytochrome b2 (Cytb2), a nuclear gene product. In order to identify the genetic lesion in this mutant we have cloned and characterized a DNA region which complements the pet ts2858 mutation. The DNA sequence revealed three open reading frames, one of which is responsible for the complementation. A 570 bp reading frame represents the structural gene PET2858, as demonstrated by in vitro mutagenesis, gene expression from a foreign promoter, and allelism tests. PET2858 encodes a 21.4 kDa protein, which is essential for growth on non-fermentable carbon sources and for the proteolytic processing of COXII and the Cytb2 intermediate. When the N-terminus of the PET2858 protein is fused to a reporter protein, the resulting hybrid molecule is imported into mitochondria. Interestingly, the N-terminal half of the deduced PET2858 protein exhibits 30.7% amino acid identity to the leader peptidase of Escherichia coli. These results suggest that PET2858 codes for a mitochondrial inner membrane protease (IMP1) or at least a subunit of it. This protease is involved in protein processing and export from the mitochondrial matrix.

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References

MORTIMER R, Schild D, Contopoulou C, Kans J . Genetic map of Saccharomyces cerevisiae, edition 10. Yeast. 1989; 5(5):321-403. DOI: 10.1002/yea.320050503. View

Nasmyth K, Reed S . Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc Natl Acad Sci U S A. 1980; 77(4):2119-23. PMC: 348663. DOI: 10.1073/pnas.77.4.2119. View

Pfanner N, Neupert W . The mitochondrial protein import apparatus. Annu Rev Biochem. 1990; 59:331-53. DOI: 10.1146/annurev.bi.59.070190.001555. View

Low B . Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968; 60(1):160-7. PMC: 539096. DOI: 10.1073/pnas.60.1.160. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Struhl K, Stinchcomb D, Scherer S, Davis R . High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci U S A. 1979; 76(3):1035-9. PMC: 383183. DOI: 10.1073/pnas.76.3.1035. View

Bohni P, Deshaies R, Schekman R . SEC11 is required for signal peptide processing and yeast cell growth. J Cell Biol. 1988; 106(4):1035-42. PMC: 2115025. DOI: 10.1083/jcb.106.4.1035. View

Schneider A, Behrens M, Scherer P, Pratje E, Michaelis G, Schatz G . Inner membrane protease I, an enzyme mediating intramitochondrial protein sorting in yeast. EMBO J. 1991; 10(2):247-54. PMC: 452640. DOI: 10.1002/j.1460-2075.1991.tb07944.x. View

Muller F, Bruhl K, Freidel K, Kowallik K, Ciriacy M . Processing of TY1 proteins and formation of Ty1 virus-like particles in Saccharomyces cerevisiae. Mol Gen Genet. 1987; 207(2-3):421-9. DOI: 10.1007/BF00331610. View

10.

Pearson W, Lipman D . Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988; 85(8):2444-8. PMC: 280013. DOI: 10.1073/pnas.85.8.2444. View

11.

Pratje E, Mannhaupt G, Michaelis G, Beyreuther K . A nuclear mutation prevents processing of a mitochondrially encoded membrane protein in Saccharomyces cerevisiae. EMBO J. 1983; 2(7):1049-54. PMC: 555233. DOI: 10.1002/j.1460-2075.1983.tb01544.x. View

12.

Daum G, Gasser S, Schatz G . Import of proteins into mitochondria. Energy-dependent, two-step processing of the intermembrane space enzyme cytochrome b2 by isolated yeast mitochondria. J Biol Chem. 1982; 257(21):13075-80. View

13.

Hartl F, Neupert W . Protein sorting to mitochondria: evolutionary conservations of folding and assembly. Science. 1990; 247(4945):930-8. DOI: 10.1126/science.2406905. View

14.

Bohni P, Daum G, Schatz G . Import of proteins into mitochondria. Partial purification of a matrix-located protease involved in cleavage of mitochondrial precursor polypeptides. J Biol Chem. 1983; 258(8):4937-43. View

15.

Zaret K, Sherman F . DNA sequence required for efficient transcription termination in yeast. Cell. 1982; 28(3):563-73. DOI: 10.1016/0092-8674(82)90211-2. View

16.

Pollock R, Hartl F, Cheng M, Ostermann J, Horwich A, Neupert W . The processing peptidase of yeast mitochondria: the two co-operating components MPP and PEP are structurally related. EMBO J. 1988; 7(11):3493-500. PMC: 454850. DOI: 10.1002/j.1460-2075.1988.tb03225.x. View

17.

Hurt E, Allison D, Muller U, Schatz G . Amino-terminal deletions in the presequence of an imported mitochondrial protein block the targeting function and proteolytic cleavage of the presequence at the carboxy terminus. J Biol Chem. 1987; 262(3):1420-4. View

18.

Halpin C, Elderfield P, James H, Zimmermann R, Dunbar B, Robinson C . The reaction specificities of the thylakoidal processing peptidase and Escherichia coli leader peptidase are identical. EMBO J. 1989; 8(12):3917-21. PMC: 402083. DOI: 10.1002/j.1460-2075.1989.tb08572.x. View

19.

Dagert M, Ehrlich S . Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979; 6(1):23-8. DOI: 10.1016/0378-1119(79)90082-9. View

20.

Messing J, Crea R, Seeburg P . A system for shotgun DNA sequencing. Nucleic Acids Res. 1981; 9(2):309-21. PMC: 326694. DOI: 10.1093/nar/9.2.309. View