Testing Odor Response Stereotypy in the Drosophila Mushroom Body

Overview

Journal Neuron

Publisher Cell Press

Specialty Neurology

Date 2008 Sep 27

PMID 18817738

Citations 97

Authors

Mala Murthy

Ila Fiete

Gilles Laurent

Affiliations

Soon will be listed here.

Abstract

The mushroom body is an insect brain structure required for olfactory learning. Its principal neurons, the Kenyon cells (KCs), form a large cell population. The neuronal populations from which their olfactory input derives (olfactory sensory and projection neurons) can be identified individually by genetic, anatomical, and physiological criteria. We ask whether KCs are similarly identifiable individually, using genetic markers and whole-cell patch-clamp in vivo. We find that across-animal responses are as diverse within the genetically labeled subset as across all KCs in a larger sample. These results combined with those from a simple model, using projection neuron odor responses as inputs, suggest that the precise circuit specification seen at earlier stages of odor processing is likely absent among the mushroom body KCs.

Citing Articles

A Critical Role for the Fascin Family of Actin Bundling Proteins in Axon Development, Brain Wiring and Function.

Hardin K, Penas A, Joubert S, Ye C, Myers K, Zheng J bioRxiv. 2025; .

PMID: 40027761 PMC: 11870622. DOI: 10.1101/2025.02.21.639554.

Neuronal wiring diagram of an adult brain.

Dorkenwald S, Matsliah A, Sterling A, Schlegel P, Yu S, McKellar C Nature. 2024; 634(8032):124-138.

PMID: 39358518 PMC: 11446842. DOI: 10.1038/s41586-024-07558-y.

Spatial constraints and cell surface molecule depletion structure a randomly connected learning circuit.

Thornton-Kolbe E, Ahmed M, Gordon F, Sieriebriennikov B, Williams D, Kurmangaliyev Y bioRxiv. 2024; .

PMID: 39071296 PMC: 11275898. DOI: 10.1101/2024.07.17.603956.

Diversity of visual inputs to Kenyon cells of the Drosophila mushroom body.

Ganguly I, Heckman E, Litwin-Kumar A, Clowney E, Behnia R Nat Commun. 2024; 15(1):5698.

PMID: 38972924 PMC: 11228034. DOI: 10.1038/s41467-024-49616-z.

What do the mushroom bodies do for the insect brain? Twenty-five years of progress.

Fiala A, Kaun K Learn Mem. 2024; 31(5).

PMID: 38862175 PMC: 11199942. DOI: 10.1101/lm.053827.123.

References

Wong A, Wang J, Axel R . Spatial representation of the glomerular map in the Drosophila protocerebrum. Cell. 2002; 109(2):229-41. DOI: 10.1016/s0092-8674(02)00707-9. View

Marin E, Jefferis G, Komiyama T, Zhu H, Luo L . Representation of the glomerular olfactory map in the Drosophila brain. Cell. 2002; 109(2):243-55. DOI: 10.1016/s0092-8674(02)00700-6. View

Perez-Orive J, Mazor O, Turner G, Cassenaer S, Wilson R, Laurent G . Oscillations and sparsening of odor representations in the mushroom body. Science. 2002; 297(5580):359-65. DOI: 10.1126/science.1070502. View

Halfon M, Gisselbrecht S, Lu J, Estrada B, Keshishian H, Michelson A . New fluorescent protein reporters for use with the Drosophila Gal4 expression system and for vital detection of balancer chromosomes. Genesis. 2002; 34(1-2):135-8. DOI: 10.1002/gene.10136. View

Bhandawat V, Olsen S, Gouwens N, Schlief M, Wilson R . Sensory processing in the Drosophila antennal lobe increases reliability and separability of ensemble odor representations. Nat Neurosci. 2007; 10(11):1474-82. PMC: 2838615. DOI: 10.1038/nn1976. View

Turner G, Bazhenov M, Laurent G . Olfactory representations by Drosophila mushroom body neurons. J Neurophysiol. 2007; 99(2):734-46. DOI: 10.1152/jn.01283.2007. View

Daniels R, Gelfand M, Collins C, DiAntonio A . Visualizing glutamatergic cell bodies and synapses in Drosophila larval and adult CNS. J Comp Neurol. 2008; 508(1):131-52. DOI: 10.1002/cne.21670. View

Tanaka N, Awasaki T, Shimada T, Ito K . Integration of chemosensory pathways in the Drosophila second-order olfactory centers. Curr Biol. 2004; 14(6):449-57. DOI: 10.1016/j.cub.2004.03.006. View

Hallem E, Ho M, Carlson J . The molecular basis of odor coding in the Drosophila antenna. Cell. 2004; 117(7):965-79. DOI: 10.1016/j.cell.2004.05.012. View

10.

Schwartz Y, Boykova T, Belyaeva E, Ashburner M, Zhimulev I . Molecular characterization of the singed wings locus of Drosophila melanogaster. BMC Genet. 2004; 5:15. PMC: 446189. DOI: 10.1186/1471-2156-5-15. View

11.

Wang Y, Guo H, Pologruto T, Hannan F, Hakker I, Svoboda K . Stereotyped odor-evoked activity in the mushroom body of Drosophila revealed by green fluorescent protein-based Ca2+ imaging. J Neurosci. 2004; 24(29):6507-14. PMC: 6729867. DOI: 10.1523/JNEUROSCI.3727-03.2004. View

12.

Schwaerzel M, Heisenberg M, Zars T . Extinction antagonizes olfactory memory at the subcellular level. Neuron. 2002; 35(5):951-60. DOI: 10.1016/s0896-6273(02)00832-2. View

13.

Ng M, Roorda R, Lima S, Zemelman B, Morcillo P, Miesenbock G . Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly. Neuron. 2002; 36(3):463-74. DOI: 10.1016/s0896-6273(02)00975-3. View

14.

Wang J, Wong A, Flores J, Vosshall L, Axel R . Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain. Cell. 2003; 112(2):271-82. DOI: 10.1016/s0092-8674(03)00004-7. View

15.

Dobritsa A, van der Goes van Naters W, Warr C, Steinbrecht R, Carlson J . Integrating the molecular and cellular basis of odor coding in the Drosophila antenna. Neuron. 2003; 37(5):827-41. DOI: 10.1016/s0896-6273(03)00094-1. View

16.

Zhu S, Chiang A, Lee T . Development of the Drosophila mushroom bodies: elaboration, remodeling and spatial organization of dendrites in the calyx. Development. 2003; 130(12):2603-10. DOI: 10.1242/dev.00466. View

17.

Strausfeld N, Sinakevitch I, Vilinsky I . The mushroom bodies of Drosophila melanogaster: an immunocytological and golgi study of Kenyon cell organization in the calyces and lobes. Microsc Res Tech. 2003; 62(2):151-69. DOI: 10.1002/jemt.10368. View

18.

Stopfer M, Jayaraman V, Laurent G . Intensity versus identity coding in an olfactory system. Neuron. 2003; 39(6):991-1004. DOI: 10.1016/j.neuron.2003.08.011. View

19.

McGuire S, Le P, Osborn A, Matsumoto K, Davis R . Spatiotemporal rescue of memory dysfunction in Drosophila. Science. 2003; 302(5651):1765-8. DOI: 10.1126/science.1089035. View

20.

Tamura T, Chiang A, Ito N, Liu H, Horiuchi J, Tully T . Aging specifically impairs amnesiac-dependent memory in Drosophila. Neuron. 2003; 40(5):1003-11. DOI: 10.1016/s0896-6273(03)00732-3. View