Chromosomal Changes Characterize Head and Neck Cancer with Poor Prognosis

Overview

Journal J Mol Med (Berl)

Specialty General Medicine

Date 2008 Sep 24

PMID 18810378

Citations 13

Authors

Verena L Bauer

Herbert Braselmann

Michael Henke

Dominik Mattern

Axel Walch

Kristian Unger

Michael Baudis

Silke Lassmann

Reinhard Huber

Johannes Wienberg

Martin Werner

Horst F Zitzelsberger

Affiliations

Soon will be listed here.

Abstract

It is well established that genetic alterations may be associated to prognosis in tumor patients. This study investigates chromosomal changes that predict the clinical outcome of head and neck squamous cell carcinoma (HNSCC) and correlate to characteristic clinicopathological parameters. We applied comparative genomic hybridization (CGH) to tissue samples from 117 HNSCC patients scheduled for radiotherapy. Genomic aberrations occurring in more than five patients were studied for impact on locoregional progression (LRP)-free survival. p values were adjusted by the Hochberg-Benjamini procedure and significant aberrations and clinical variables subjected to a stepwise backwards Cox proportional model. Significant alterations were further analyzed by array-CGH and fluorescence in situ hybridization (FISH). In multivariate survival analysis gains on 1q and 16q predict reduced LRP-free survival independently from known prognostic factors. Cluster analysis separated the HNSCC cases into two groups (cluster 1 and 2) that are characterized by significant differences for imbalances in 13 chromosomal regions. Moreover, it became apparent that cluster 1 correlates to nonanemic patients, while cluster 2 represents predominantly anemic cases. Array-CGH pinpoints 16q24.3 to be the region of interest on chromosome 16 which was further verified by FISH analysis where an increased copy number of FANCA, a member of the Fanconi anemia/breast cancer pathway, could be identified. This study demonstrates that chromosomal gains on 1q and 16q as well as chromosomal loss on 18q represent prognostic markers in HNSCC and that these alterations may explain to some extent the dismal course of a subgroup of patients.

Citing Articles

(Cyto)genomic and epigenetic characterization of BICR 10 cell line and three new established primary human head and neck squamous cell carcinoma cultures.

Ribeiro I, Rodrigues J, Mascarenhas A, Marques V, Caramelo F, Juliao M Genes Genomics. 2019; 41(10):1207-1221.

PMID: 31321735 DOI: 10.1007/s13258-019-00850-6.

A prognostic mRNA expression signature of four 16q24.3 genes in radio(chemo)therapy-treated head and neck squamous cell carcinoma (HNSCC).

Wintergerst L, Selmansberger M, Maihoefer C, Schuttrumpf L, Walch A, Wilke C Mol Oncol. 2018; 12(12):2085-2101.

PMID: 30259648 PMC: 6275282. DOI: 10.1002/1878-0261.12388.

Genomic profile of oral squamous cell carcinomas with an adjacent leukoplakia or with an erythroleukoplakia that evolved after the treatment of primary tumor: A report of two cases.

Ribeiro I, Marques F, Barroso L, Rodrigues J, Caramelo F, Melo J Mol Med Rep. 2017; 16(5):6780-6786.

PMID: 28901451 PMC: 5865835. DOI: 10.3892/mmr.2017.7428.

Initial evidence for Sec62 as a prognostic marker in advanced head and neck squamous cell carcinoma.

Wemmert S, Lindner Y, Linxweiler J, Wagenpfeil S, Bohle R, Niewald M Oncol Lett. 2016; 11(3):1661-1670.

PMID: 26998059 PMC: 4774472. DOI: 10.3892/ol.2016.4135.

Genetic variants in fanconi anemia pathway genes BRCA2 and FANCA predict melanoma survival.

Yin J, Liu H, Liu Z, Wang L, Chen W, Zhu D J Invest Dermatol. 2014; 135(2):542-550.

PMID: 25243787 PMC: 4289462. DOI: 10.1038/jid.2014.416.

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