Super-resolution Imaging in Live Caulobacter Crescentus Cells Using Photoswitchable EYFP

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2008 Sep 17

PMID 18794860

Citations 135

Authors

Julie S Biteen

Michael A Thompson

Nicole K Tselentis

Grant R Bowman

Lucy Shapiro

W E Moerner

Affiliations

Soon will be listed here.

Abstract

The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.

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