Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes

Overview

Journal J Phys Chem B

Publisher American Chemical Society

Specialty Chemistry

Date 2021 Nov 24

PMID 34818013

Authors

Yusuf Qutbuddin

Jan-Hagen Krohn

Gereon A Bruggenthies

Johannes Stein

Svetozar Gavrilovic

Florian Stehr

Petra Schwille

Affiliations

Soon will be listed here.

Abstract

Nanotechnology often exploits DNA origami nanostructures assembled into even larger superstructures up to micrometer sizes with nanometer shape precision. However, large-scale assembly of such structures is very time-consuming. Here, we investigated the efficiency of superstructure assembly on surfaces using indirect cross-linking through low-complexity connector strands binding staple strand extensions, instead of connector strands binding to scaffold loops. Using single-molecule imaging techniques, including fluorescence microscopy and atomic force microscopy, we show that low sequence complexity connector strands allow formation of DNA origami superstructures on lipid membranes, with an order-of-magnitude enhancement in the assembly speed of superstructures. A number of effects, including suppression of DNA hairpin formation, high local effective binding site concentration, and multivalency are proposed to contribute to the acceleration. Thus, the use of low-complexity sequences for DNA origami higher-order assembly offers a very simple but efficient way of improving throughput in DNA origami design.

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