Pigs As Source of Methicillin-resistant Staphylococcus Aureus CC398 Infections in Humans, Denmark

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2008 Sep 2

PMID 18760004

Citations 98

Authors

Hannah C Lewis

Kare Molbak

Catrin Reese

Frank M Aarestrup

Mette Selchau

Marit Sorum

Robert L Skov

Affiliations

Soon will be listed here.

Abstract

An emerging subtype of methicillin-resistant Staphylococcus aureus (MRSA), clonal complex (CC) 398, is associated with animals, particularly pigs. We conducted a matched case-control and a case-case study comparing 21 CC398 case-patients with 2 controls randomly selected from the Danish Civil Registry and 2 case-patients infected with MRSA other than CC398. On farms of case-patients, animals were examined for MRSA. Thirteen case-patients reported pig exposure. Living or working on farms with animals was an independent risk factor for CC398 in the case-control (matched odds ratio [MOR] 35.4, 95% confidence interval [CI] 2.7-469.8) and the case-case study (MOR 14.5, 95%CI 2.7-76.7). History of hospitalization was associated with an increased risk only in the case-control study (MOR 11.4, 95% CI 1.4-94.8). A total of 23 of 50 pigs on 4 of 5 farms were positive for CC398. Our results, corroborated by microbiologic testing, demonstrate that pigs are a source of CC398 in Denmark.

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References

Kwon N, Park K, Moon J, Jung W, Kim S, Kim J . Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea. J Antimicrob Chemother. 2005; 56(4):624-32. DOI: 10.1093/jac/dki306. View

Lee J . Methicillin (Oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl Environ Microbiol. 2003; 69(11):6489-94. PMC: 262320. DOI: 10.1128/AEM.69.11.6489-6494.2003. View

Ruhlmann C, Kolmos H, Kristiansen J, Skov R . [Pigs as an infection source for methicillin resistant Staphylococcus aureus infections in humans]. Ugeskr Laeger. 2008; 170(43):3436. View

Aubry-Damon H, Grenet K, Sall-Ndiaye P, Che D, Cordeiro E, Bougnoux M . Antimicrobial resistance in commensal flora of pig farmers. Emerg Infect Dis. 2004; 10(5):873-9. PMC: 3323198. DOI: 10.3201/eid1005.030735. View

Oliveira D, de Lencastre H . Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2002; 46(7):2155-61. PMC: 127318. DOI: 10.1128/AAC.46.7.2155-2161.2002. View

Ekkelenkamp M, Sekkat M, Carpaij N, Troelstra A, Bonten M . [Endocarditis due to meticillin-resistant Staphylococcus aureus originating from pigs]. Ned Tijdschr Geneeskd. 2006; 150(44):2442-7. View

Armand-Lefevre L, Ruimy R, Andremont A . Clonal comparison of Staphylococcus aureus isolates from healthy pig farmers, human controls, and pigs. Emerg Infect Dis. 2005; 11(5):711-4. PMC: 3320358. DOI: 10.3201/eid1105.040866. View

A Faria N, Oliveira D, Westh H, Monnet D, Larsen A, Skov R . Epidemiology of emerging methicillin-resistant Staphylococcus aureus (MRSA) in Denmark: a nationwide study in a country with low prevalence of MRSA infection. J Clin Microbiol. 2005; 43(4):1836-42. PMC: 1081382. DOI: 10.1128/JCM.43.4.1836-1842.2005. View

Wulf M, Sorum M, van Nes A, Skov R, Melchers W, Klaassen C . Prevalence of methicillin-resistant Staphylococcus aureus among veterinarians: an international study. Clin Microbiol Infect. 2007; 14(1):29-34. DOI: 10.1111/j.1469-0691.2007.01873.x. View

10.

Harmsen D, Claus H, Witte W, Rothganger J, Claus H, Turnwald D . Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol. 2003; 41(12):5442-8. PMC: 309029. DOI: 10.1128/JCM.41.12.5442-5448.2003. View

11.

Hammerum A, Heuer O, Emborg H, Bagger-Skjot L, Jensen V, Rogues A . Danish integrated antimicrobial resistance monitoring and research program. Emerg Infect Dis. 2008; 13(11):1632-9. PMC: 3375779. DOI: 10.3201/eid1311.070421. View

12.

Normanno G, Corrente M, La Salandra G, DAmbrosio A, Quaglia N, Parisi A . Methicillin-resistant Staphylococcus aureus (MRSA) in foods of animal origin product in Italy. Int J Food Microbiol. 2007; 117(2):219-22. DOI: 10.1016/j.ijfoodmicro.2007.04.006. View

13.

Lina G, Piemont Y, Bes M, Peter M, Gauduchon V, Vandenesch F . Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis. 1999; 29(5):1128-32. DOI: 10.1086/313461. View

14.

Witte W, Strommenger B, Stanek C, Cuny C . Methicillin-resistant Staphylococcus aureus ST398 in humans and animals, Central Europe. Emerg Infect Dis. 2007; 13(2):255-8. PMC: 2725865. DOI: 10.3201/eid1302.060924. View

15.

Devriese L, OEDING P . Characteristics of Staphylococcus aureus strains isolated from different animal species. Res Vet Sci. 1976; 21(3):284-91. View

16.

Mehrotra M, Wang G, Johnson W . Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J Clin Microbiol. 2000; 38(3):1032-5. PMC: 86330. DOI: 10.1128/JCM.38.3.1032-1035.2000. View

17.

Sergio D, Koh T, Hsu L, Ogden B, Goh A, Chow P . Investigation of meticillin-resistant Staphylococcus aureus in pigs used for research. J Med Microbiol. 2007; 56(Pt 8):1107-1109. DOI: 10.1099/jmm.0.47283-0. View

18.

McCarthy N, Giesecke J . Case-case comparisons to study causation of common infectious diseases. Int J Epidemiol. 1999; 28(4):764-8. DOI: 10.1093/ije/28.4.764. View

19.

Scanvic A, Denic L, Gaillon S, Giry P, Andremont A, Lucet J . Duration of colonization by methicillin-resistant Staphylococcus aureus after hospital discharge and risk factors for prolonged carriage. Clin Infect Dis. 2001; 32(10):1393-8. DOI: 10.1086/320151. View

20.

Khanna T, Friendship R, Dewey C, Weese J . Methicillin resistant Staphylococcus aureus colonization in pigs and pig farmers. Vet Microbiol. 2007; 128(3-4):298-303. DOI: 10.1016/j.vetmic.2007.10.006. View