A Conserved Steroid Binding Site in Cytochrome C Oxidase

Overview

Journal Biochemistry

Specialty Biochemistry

Date 2008 Sep 2

PMID 18759498

Citations 27

Authors

Ling Qin

Denise A Mills

Leann Buhrow

Carrie Hiser

Shelagh Ferguson-Miller

Affiliations

Soon will be listed here.

Abstract

Micromolar concentrations of the bile salt deoxycholate are shown to rescue the activity of an inactive mutant, E101A, in the K proton pathway of Rhodobacter sphaeroides cytochrome c oxidase. A crystal structure of the wild-type enzyme reveals, as predicted, deoxycholate bound with its carboxyl group at the entrance of the K path. Since cholate is a known potent inhibitor of bovine oxidase and is seen in a similar position in the bovine structure, the crystallographically defined, conserved steroid binding site could reveal a regulatory site for steroids or structurally related molecules that act on the essential K proton path.

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