In Vitro Differentiation of Human Monocytes into Dendritic Cells by Peptic-tryptic Digest of Gliadin is Independent of Genetic Predisposition and the Presence of Celiac Disease

Overview

Journal J Clin Immunol

Publisher Springer

Specialty Allergy & Immunology

Date 2008 Aug 13

PMID 18696220

Citations 13

Authors

Maryam Rakhimova

Birgit Esslinger

Anja Schulze-Krebs

Eckhart G Hahn

Detlef Schuppan

Walburga Dieterich

Affiliations

Soon will be listed here.

Abstract

Introduction: This study was done to further reveal the role of the innate immune system in celiac disease.

Methods: Dendritic cells were matured from venous blood of patients with active or treated celiac disease and DQ2-DQ8-positive or negative controls. Dendritic cells were treated with a peptic-tryptic digest of gliadin (500 microg/ml) and their activation was analyzed by fluorescent-activated cell sorting analysis, cytokine secretion, and their ability to elicit T cell proliferation.

Results And Discussion: Gliadin upregulated interleukin (IL)-6, IL-8, and IL-12 (p40) secretion in dendritic cells and induced strong expression of the maturation markers human leukocyte antigen (HLA)-DR, CD25, CD83, and CD86 of all subjects irrespective of their genotype or the presence of disease, whereas the digest of bovine serum albumin showed no effect. However, gliadin-stimulated dendritic cells from active celiac showed enhanced stimulation of autologous T cells compared to the other groups.

Conclusion: Further research should be aimed at identifying the mechanisms that control inflammation in healthy individuals.

Citing Articles

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Intestinal Epithelium Modulates Macrophage Response to Gliadin in Celiac Disease.

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