Revealing the Role of Phosphatidylserine in Shear Stress-mediated Protection in Endothelial Cells

Overview

Journal Endothelium

Publisher Informa Healthcare

Specialty Cell Biology

Date 2008 Jul 30

PMID 18663626

Citations 2

Authors

Julie K Freed

Michael R Shortreed

Christopher J Kleefisch

Lloyd M Smith

Andrew S Greene

Affiliations

Soon will be listed here.

Abstract

Previous studies have demonstrated that endothelial cells exposed to laminar shear stress are protected from apoptotic stimuli such as tumor necrosis factor (TNF)-alpha. The authors investigated the role of phosphatidylserine (PS) in this phenomenon. Western blot analysis of cleaved caspase 3 was used as an indicator of apoptosis and revealed that in the absence of serine, endothelial cells exposed to laminar shear stress were unable to protect against TNF-alpha-induced apoptosis, in contrast to sheared cells grown in regular medium. It was also found that shear-induced activation of the Akt pathway was significantly decreased in cells grown without serine. In addition, quantitation of PS using a novel isotopic labeling technique involving the use of formalin revealed that stearoyl-oleic PS (18:0/18:1) did not increase during shear treatment. These findings suggest that basal levels of PS are required to activate survival pathways in endothelial cells and thereby contribute to the overall protective mechanism initiated by shear stress.

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