Expression of Carboxylesterase and Lipase Genes in Rat Liver Cell-types

Overview

Journal Biochem Biophys Res Commun

Publisher Elsevier

Specialty Biochemistry

Date 2008 Jul 22

PMID 18639528

Citations 30

Authors

Tommaso Mello

Alice Nakatsuka

Sharry Fears

Wilhelmina Davis

Hidekazu Tsukamoto

William F Bosron

Sonal P Sanghani

Affiliations

Soon will be listed here.

Abstract

Approximately 80% of the body vitamin A is stored in liver stellate cells with in the lipid droplets as retinyl esters. In low vitamin A status or after liver injury, stellate cells are depleted of the stored retinyl esters by their hydrolysis to retinol. However, the identity of retinyl ester hydrolase(s) expressed in stellate cells is unknown. The expression of carboxylesterase and lipase genes in purified liver cell-types was investigated by real-time PCR. We found that six carboxylesterase and hepatic lipase genes were expressed in hepatocytes. Adipose triglyceride lipase was expressed in Kupffer cells, stellate cells and endothelial cells. Lipoprotein lipase expression was detected in Kupffer cells and stellate cells. As a function of stellate cell activation, expression of adipose triglyceride lipase decreased by twofold and lipoprotein lipase increased by 32-fold suggesting that it may play a role in retinol ester hydrolysis during stellate cell activation.

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References

Lombardo D, Guy O . Studies on the substrate specificity of a carboxyl ester hydrolase from human pancreatic juice. II. Action on cholesterol esters and lipid-soluble vitamin esters. Biochim Biophys Acta. 1980; 611(1):147-55. DOI: 10.1016/0005-2744(80)90050-9. View

Mentlein R, HEYMANN E . Hydrolysis of retinyl esters by non-specific carboxylesterases from rat liver endoplasmic reticulum. Biochem J. 1987; 245(3):863-7. PMC: 1148208. DOI: 10.1042/bj2450863. View

Blaner W, Obunike J, Kurlandsky S, Piantedosi R, Deckelbaum R, Goldberg I . Lipoprotein lipase hydrolysis of retinyl ester. Possible implications for retinoid uptake by cells. J Biol Chem. 1994; 269(24):16559-65. View

Blaner W, Dixon J, Moriwaki H, Martino R, Stein O, STEIN Y . Studies on the in vivo transfer of retinoids from parenchymal to stellate cells in rat liver. Eur J Biochem. 1987; 164(2):301-7. DOI: 10.1111/j.1432-1033.1987.tb11058.x. View

Hendriks H, Brouwer A, Knook D . Isolation, purification, and characterization of liver cell types. Methods Enzymol. 1990; 190:49-58. DOI: 10.1016/0076-6879(90)90008-o. View

Lee P, Sladek R, Greenwood C, Hudson T . Control genes and variability: absence of ubiquitous reference transcripts in diverse mammalian expression studies. Genome Res. 2002; 12(2):292-7. PMC: 155273. DOI: 10.1101/gr.217802. View

Van Bennekum A, Kako Y, Weinstock P, Harrison E, Deckelbaum R, Goldberg I . Lipoprotein lipase expression level influences tissue clearance of chylomicron retinyl ester. J Lipid Res. 1999; 40(3):565-74. View

Blaner W, Hendriks H, Brouwer A, de Leeuw A, Knook D, Goodman D . Retinoids, retinoid-binding proteins, and retinyl palmitate hydrolase distributions in different types of rat liver cells. J Lipid Res. 1985; 26(10):1241-51. View

Van Bennekum A, Li L, Piantedosi R, Shamir R, Vogel S, Fisher E . Carboxyl ester lipase overexpression in rat hepatoma cells and CEL deficiency in mice have no impact on hepatic uptake or metabolism of chylomicron-retinyl ester. Biochemistry. 1999; 38(13):4150-6. DOI: 10.1021/bi981680+. View

10.

Blomhoff R, Berg T, Norum K . Transfer of retinol from parenchymal to stellate cells in liver is mediated by retinol-binding protein. Proc Natl Acad Sci U S A. 1988; 85(10):3455-8. PMC: 280230. DOI: 10.1073/pnas.85.10.3455. View

11.

Kershaw E, Schupp M, Guan H, Gardner N, Lazar M, Flier J . PPARgamma regulates adipose triglyceride lipase in adipocytes in vitro and in vivo. Am J Physiol Endocrinol Metab. 2007; 293(6):E1736-45. PMC: 2819189. DOI: 10.1152/ajpendo.00122.2007. View

12.

Mead J, Irvine S, Ramji D . Lipoprotein lipase: structure, function, regulation, and role in disease. J Mol Med (Berl). 2002; 80(12):753-69. DOI: 10.1007/s00109-002-0384-9. View

13.

Reeves H, Friedman S . Activation of hepatic stellate cells--a key issue in liver fibrosis. Front Biosci. 2002; 7:d808-26. DOI: 10.2741/reeves. View

14.

Wei S, Lai K, Patel S, Piantedosi R, Shen H, Colantuoni V . Retinyl ester hydrolysis and retinol efflux from BFC-1beta adipocytes. J Biol Chem. 1997; 272(22):14159-65. DOI: 10.1074/jbc.272.22.14159. View

15.

Semenkovich C, Chen S, Wims M, Luo C, Li W, Chan L . Lipoprotein lipase and hepatic lipase mRNA tissue specific expression, developmental regulation, and evolution. J Lipid Res. 1989; 30(3):423-31. View

16.

Sanghani S, Davis W, Dumaual N, Mahrenholz A, Bosron W . Identification of microsomal rat liver carboxylesterases and their activity with retinyl palmitate. Eur J Biochem. 2002; 269(18):4387-98. DOI: 10.1046/j.1432-1033.2002.03121.x. View

17.

Sun G, Alexson S, Harrison E . Purification and characterization of a neutral, bile salt-independent retinyl ester hydrolase from rat liver microsomes. Relationship To rat carboxylesterase ES-2. J Biol Chem. 1997; 272(39):24488-93. DOI: 10.1074/jbc.272.39.24488. View

18.

Friedman S, Roll F . Isolation and culture of hepatic lipocytes, Kupffer cells, and sinusoidal endothelial cells by density gradient centrifugation with Stractan. Anal Biochem. 1987; 161(1):207-18. DOI: 10.1016/0003-2697(87)90673-7. View

19.

Friedman S . Molecular mechanisms of hepatic fibrosis and principles of therapy. J Gastroenterol. 1997; 32(3):424-30. DOI: 10.1007/BF02934504. View

20.

Haemmerle G, Zimmermann R, Hayn M, Theussl C, Waeg G, Wagner E . Hormone-sensitive lipase deficiency in mice causes diglyceride accumulation in adipose tissue, muscle, and testis. J Biol Chem. 2001; 277(7):4806-15. DOI: 10.1074/jbc.M110355200. View