Monitoring Serial Changes in Circulating Human Breast Cancer Cells in Murine Xenograft Models

Overview

Journal Cancer Res

Specialty Oncology

Date 2008 Jul 18

PMID 18632603

Citations 27

Authors

Jean-Pierre Eliane

Madeline Repollet

Kathryn E Luker

Martha Brown

James M Rae

Gabriela Dontu

Anne F Schott

Max Wicha

Gerald V Doyle

Daniel F Hayes

Gary D Luker

Affiliations

Soon will be listed here.

Abstract

Circulating tumor cells (CTC) are emerging as a powerful prognostic and predictive biomarker in several types of cancer, including breast, colon, and prostate. Studies of CTC in metastasis and further development of CTC as a biomarker in cancer have been limited by the inability to repetitively monitor CTC in mouse models of cancer. We have validated a method to enumerate CTC in blood samples obtained from living mice using a modified version of an in vitro diagnostic system for quantifying CTC in patients. Different routes of blood collection were tested to identify a method to reproducibly recover CTC from tumor-bearing mice without interference from contaminating normal murine epithelial cells. CTC are present in blood samples from mice bearing orthotopic xenografts of several different breast cancer cell lines and primary breast cancer cells from patient biopsies. We also show that this technology can be used for serial monitoring of CTC in mouse xenograft models of human breast cancer. These results establish a new method for studying CTC in mouse models of epithelial cancer, providing the foundation for studies of molecular regulation of CTC in cancer and CTC as biomarker for therapeutic efficacy.

Citing Articles

Mobilization of Circulating Tumor Cells after Short- and Long-Term FOLFIRINOX and GEM/nab-PTX Chemotherapy in Xenograft Mouse Models of Human Pancreatic Cancer.

Ito Y, Kobuchi S, Kawakita A, Tosaka K, Matsunaga Y, Yoshioka S Cancers (Basel). 2023; 15(22).

PMID: 38001741 PMC: 10670901. DOI: 10.3390/cancers15225482.

The functional and clinical roles of liquid biopsy in patient-derived models.

Zhu Z, Hu E, Shen H, Tan J, Zeng S J Hematol Oncol. 2023; 16(1):36.

PMID: 37031172 PMC: 10082989. DOI: 10.1186/s13045-023-01433-5.

Basic Science with Preclinical Models to Investigate and Develop Liquid Biopsy: What Are the Available Data and Is It a Fruitful Approach?.

Cena B, Melloul E, Demartines N, Dormond O, Labgaa I Int J Mol Sci. 2022; 23(10).

PMID: 35628154 PMC: 9141279. DOI: 10.3390/ijms23105343.

Investigating circulating tumor cells and distant metastases in patient-derived orthotopic xenograft models of triple-negative breast cancer.

Ramani V, Lemaire C, Triboulet M, Casey K, Heirich K, Renier C Breast Cancer Res. 2019; 21(1):98.

PMID: 31462307 PMC: 6714238. DOI: 10.1186/s13058-019-1182-4.

The Detection and Morphological Analysis of Circulating Tumor and Host Cells in Breast Cancer Xenograft Models.

Cleris L, Daidone M, Fina E, Cappelletti V Cells. 2019; 8(7).

PMID: 31284534 PMC: 6679018. DOI: 10.3390/cells8070683.

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