Analysis of Skeletal Muscle Function in the C57BL6/SV129 Syncoilin Knockout Mouse
Overview
Affiliations
Syncoilin is a 64-kDa intermediate filament protein expressed in skeletal muscle and enriched at the perinucleus, sarcolemma, and myotendinous and neuromuscular junctions. Due to its pattern of cellular localization and binding partners, syncoilin is an ideal candidate to be both an important structural component of myocytes and a potential mediator of inherited myopathies. Here we present a report of a knockout mouse model for syncoilin and the results of an investigation into the effect of a syncoilin null state on striated muscle function in 6-8-week-old mice. An analysis of proteins known to associate with syncoilin showed that ablation of syncoilin had no effect on absolute expression or spatial localization of desmin or alpha dystrobrevin. Our syncoilin-null animal exhibited no differences in cardiotoxin-induced muscle regeneration, voluntary wheel running, or enforced treadmill exercise capacity, relative to wild-type controls. Finally, a mechanical investigation of isolated soleus and extensor digitorum longus indicated a potential differential reduction in muscle strength and resilience. We are the first to present data identifying an increased susceptibility to muscle damage in response to an extended forced exercise regime in syncoilin-deficient muscle. This study establishes a second viable syncoilin knockout model and highlights the importance of further investigations to determine the role of syncoilin in skeletal muscle.
Hassani M, Moutachi D, Lemaitre M, Boulinguiez A, Furling D, Agbulut O PLoS One. 2024; 19(3):e0295700.
PMID: 38457407 PMC: 10923407. DOI: 10.1371/journal.pone.0295700.
Skeletal Muscle Regeneration in Cardiotoxin-Induced Muscle Injury Models.
Wang Y, Lu J, Liu Y Int J Mol Sci. 2022; 23(21).
PMID: 36362166 PMC: 9657523. DOI: 10.3390/ijms232113380.
Klymkowsky M F1000Res. 2019; 8.
PMID: 31602295 PMC: 6774051. DOI: 10.12688/f1000research.19950.1.
In vivo generation of a mature and functional artificial skeletal muscle.
Fuoco C, Rizzi R, Biondo A, Longa E, Mascaro A, Shapira-Schweitzer K EMBO Mol Med. 2015; 7(4):411-22.
PMID: 25715804 PMC: 4403043. DOI: 10.15252/emmm.201404062.
Lee E, Malik A, Pokharel S, Ahmad S, Mir B, Cho K PLoS One. 2014; 9(3):e92447.
PMID: 24647404 PMC: 3960249. DOI: 10.1371/journal.pone.0092447.