Nef Modulates the Immunogenicity of Gag Encoded in a Non-infectious HIV DNA Vaccine

Overview

Journal Vaccine

Specialty Allergy & Immunology

Date 2008 Jul 1

PMID 18586360

Citations 5

Authors

Geraldine Arrode

Ramakrishna Hegde

Yuhuai Jin

Dinesh Kumar Singh

Opendra Narayan

Yahia Chebloune

Affiliations

Soon will be listed here.

Abstract

Gag-CD8+ T cell responses are associated with immune control of HIV infection. Since during HIV infection Nef impairs T cell responses, we evaluated whether deletion of nef from a non-infectious HIV DNA vaccine (Delta4 Nef+), creating Delta5 Nef(-), would affect its immunogenicity. When compared with Delta4, mice injected with Delta5 developed significantly lower CD8+ T cell responses to Gag, but no significant change in the responses to Env was observed. In vitro, deletion of Nef abrogated the induced cell death, production of virus-like particles and release of Gag from transfected cells. Thus, the effect of Nef in causing extrusion of Gag might adjuvant the CD8+ T cell responses to Gag in DNA vaccine.

Citing Articles

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