Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-sensing Movements

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2008 Jun 26

PMID 18575613

Citations 85

Authors

Alicia Lundby

Hiroki Mutoh

Dimitar Dimitrov

Walther Akemann

Thomas Knopfel

Affiliations

Soon will be listed here.

Abstract

Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.

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