Exaggerated Expression of Skeletal Muscle-derived Interleukin-6, but Not TNFalpha, in Mice Lacking Interleukin-10

Overview

Journal J Neuroimmunol

Specialty Neurology

Date 2008 Jun 21

PMID 18565597

Citations 8

Authors

Kimberly A Huey

Robert H McCusker

Keith W Kelley

Affiliations

Soon will be listed here.

Abstract

IL-10 reduces cytokine expression in non-muscle tissues, but its effect on skeletal muscle remains undefined. Therefore, we tested the hypothesis that endogenous IL-10 acts to reduce cytokines in the gastrocnemius muscle by comparing IL-6 and TNFalpha expression in wild-type (IL-10(+/+)) and IL-10 deficient (IL-10(-/-)) mice following an inflammatory insult induced by peripheral LPS. IL-6 mRNA expression increased following LPS for both IL-10(+/+) and IL-10(-/-) mice; the response was greater and prolonged in IL-10(-/-) mice. Muscle TNFalpha mRNA also increased, but without differences between genotypes. IL-6 protein concentrations were elevated by LPS with a greater and prolonged response for IL-10(-/-) mice, but TNFalpha did not change. These results provide the first in vivo evidence that endogenous IL-10 attenuates IL-6 expression by skeletal muscle in response to LPS.

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