Molecular Genetic Mining of the Aspergillus Secondary Metabolome: Discovery of the Emericellamide Biosynthetic Pathway

Overview

Journal Chem Biol

Publisher Elsevier

Specialties Biochemistry
Biology
Chemistry

Date 2008 Jun 19

PMID 18559263

Citations 90

Authors

Yi-Ming Chiang

Edyta Szewczyk

Tania Nayak

Ashley D Davidson

James F Sanchez

Hsien-Chun Lo

Wen-Yueh Ho

Hagop Simityan

Eric Kuo

Alex Praseuth

Kenji Watanabe

Berl R Oakley

Clay C C Wang

Affiliations

Soon will be listed here.

Abstract

The recently sequenced genomes of several Aspergillus species have revealed that these organisms have the potential to produce a surprisingly large range of natural products, many of which are currently unknown. We have found that A. nidulans produces emericellamide A, an antibiotic compound of mixed origins with polyketide and amino acid building blocks. Additionally, we describe the discovery of four previously unidentified, related compounds that we designate emericellamide C-F. Using recently developed gene targeting techniques, we have identified the genes involved in emericellamide biosynthesis. The emericellamide gene cluster contains one polyketide synthase and one nonribosomal peptide synthetase. From the sequences of the genes, we are able to deduce a biosynthetic pathway for the emericellamides. The identification of this biosynthetic pathway opens the door to engineering novel analogs of this structurally complex metabolite.

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