Single-headed Mode of Kinesin-5

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2008 Jun 17

PMID 18552767

Citations 13

Authors

Kuniyoshi Kaseda

Isabelle Crevel

Keiko Hirose

Robert A Cross

Affiliations

Soon will be listed here.

Abstract

In most organisms, kinesin-5 motors are essential for mitosis and meiosis, where they crosslink and slide apart the antiparallel microtubule half-spindles. Recently, it was shown using single-molecule optical trapping that a truncated, double-headed human kinesin-5 dimer can step processively along microtubules. However, processivity is limited ( approximately 8 steps) with little coordination between the heads, raising the possibility that kinesin-5 motors might also be able to move by a nonprocessive mechanism. To investigate this, we engineered single-headed kinesin-5 dimers. We show that a set of these single-headed Eg5 dimers drive microtubule sliding at about 90% of wild-type velocity, indicating that Eg5 can slide microtubules by a mechanism in which one head of each Eg5 head-pair is effectively redundant. On the basis of this, we propose a muscle-like model for Eg5-driven microtubule sliding in spindles in which most force-generating events are single-headed interactions and alternate-heads processivity is rare.

Citing Articles

Drug resistance dependent on allostery: A P-loop rigor Eg5 mutant exhibits resistance to allosteric inhibition by STLC.

Indorato R, DeBonis S, Garcia-Saez I, Skoufias D Front Oncol. 2022; 12:965455.

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Cryo-EM structure of a microtubule-bound parasite kinesin motor and implications for its mechanism and inhibition.

Cook A, Roberts A, Atherton J, Tewari R, Topf M, Moores C J Biol Chem. 2021; 297(5):101063.

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Mechanisms by Which Kinesin-5 Motors Perform Their Multiple Intracellular Functions.

Pandey H, Popov M, Goldstein-Levitin A, Gheber L Int J Mol Sci. 2021; 22(12).

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Molecular cloning, expression, overproduction and characterization of human TRAIP Leucine zipper protein.

Bhat E, Sajjad N, Sabir J, Kamli M, Hakeem K, Rather I Saudi J Biol Sci. 2020; 27(6):1562-1565.

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These motors were made for walking.

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