Loop-mediated Isothermal Amplification Assay for Rapid Detection of Common Strains of Escherichia Coli

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2008 Jun 14

PMID 18550738

Citations 89

Authors

Joshua Hill

Shilpa Beriwal

Ishwad Chandra

Vinod K Paul

Aarti Kapil

Tripti Singh

Robert M Wadowsky

Vinita Singh

Ankur Goyal

Timo Jahnukainen

James R Johnson

Phillip I Tarr

Abhay Vats

Affiliations

Soon will be listed here.

Abstract

We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.

Citing Articles

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References

Hara-Kudo Y, Nemoto J, Ohtsuka K, Segawa Y, Takatori K, Kojima T . Sensitive and rapid detection of Vero toxin-producing Escherichia coli using loop-mediated isothermal amplification. J Med Microbiol. 2007; 56(Pt 3):398-406. DOI: 10.1099/jmm.0.46819-0. View

Trotman H, Bell Y . Neonatal sepsis in very low birthweight infants at the University Hospital of the West indies. West Indian Med J. 2006; 55(3):165-9. DOI: 10.1590/s0043-31442006000300007. View

Song T, Toma C, Nakasone N, Iwanaga M . Sensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop-mediated isothermal amplification method. FEMS Microbiol Lett. 2005; 243(1):259-63. DOI: 10.1016/j.femsle.2004.12.014. View

Ochman H, Selander R . Standard reference strains of Escherichia coli from natural populations. J Bacteriol. 1984; 157(2):690-3. PMC: 215307. DOI: 10.1128/jb.157.2.690-693.1984. View

Bista B, Ishwad C, Wadowsky R, Manna P, Randhawa P, Gupta G . Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus. J Clin Microbiol. 2007; 45(5):1581-7. PMC: 1865893. DOI: 10.1128/JCM.01024-06. View

Leung A, Robson W, Davies H . Traveler's diarrhea. Adv Ther. 2006; 23(4):519-27. DOI: 10.1007/BF02850040. View

Klein E, Boster D, Stapp J, Wells J, Qin X, Clausen C . Diarrhea etiology in a Children's Hospital Emergency Department: a prospective cohort study. Clin Infect Dis. 2006; 43(7):807-13. DOI: 10.1086/507335. View

Behzadbehbahani A, Klapper P, Vallely P, Cleator G . Detection of BK virus in urine by polymerase chain reaction: a comparison of DNA extraction methods. J Virol Methods. 1997; 67(2):161-6. DOI: 10.1016/s0166-0934(97)00101-8. View

Chernesky M, Jang D, Sellors J, Luinstra K, Chong S, Castriciano S . Urinary inhibitors of polymerase chain reaction and ligase chain reaction and testing of multiple specimens may contribute to lower assay sensitivities for diagnosing Chlamydia trachomatis infected women. Mol Cell Probes. 1997; 11(4):243-9. DOI: 10.1006/mcpr.1997.0109. View

10.

Shaikh N, Tarr P . Escherichia coli O157:H7 Shiga toxin-encoding bacteriophages: integrations, excisions, truncations, and evolutionary implications. J Bacteriol. 2003; 185(12):3596-605. PMC: 156235. DOI: 10.1128/JB.185.12.3596-3605.2003. View

11.

Toye B, Woods W, Bobrowska M, Ramotar K . Inhibition of PCR in genital and urine specimens submitted for Chlamydia trachomatis testing. J Clin Microbiol. 1998; 36(8):2356-8. PMC: 105049. DOI: 10.1128/JCM.36.8.2356-2358.1998. View

12.

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N . Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000; 28(12):E63. PMC: 102748. DOI: 10.1093/nar/28.12.e63. View

13.

Bonacorsi S, Bingen E . Molecular epidemiology of Escherichia coli causing neonatal meningitis. Int J Med Microbiol. 2005; 295(6-7):373-81. DOI: 10.1016/j.ijmm.2005.07.011. View

14.

Clarke S . Diarrhoeagenic Escherichia coli--an emerging problem?. Diagn Microbiol Infect Dis. 2001; 41(3):93-8. DOI: 10.1016/s0732-8893(01)00303-0. View

15.

Yano A, Ishimaru R, Hujikata R . Rapid and sensitive detection of heat-labile I and heat-stable I enterotoxin genes of enterotoxigenic Escherichia coli by Loop-Mediated Isothermal Amplification. J Microbiol Methods. 2006; 68(2):414-20. DOI: 10.1016/j.mimet.2006.09.024. View

16.

Khan G, Kangro H, Coates P, Heath R . Inhibitory effects of urine on the polymerase chain reaction for cytomegalovirus DNA. J Clin Pathol. 1991; 44(5):360-5. PMC: 496862. DOI: 10.1136/jcp.44.5.360. View

17.

Farthing M . Bugs and the gut: an unstable marriage. Best Pract Res Clin Gastroenterol. 2004; 18(2):233-9. DOI: 10.1016/j.bpg.2003.11.001. View

18.

Russo T, Johnson J . Medical and economic impact of extraintestinal infections due to Escherichia coli: focus on an increasingly important endemic problem. Microbes Infect. 2003; 5(5):449-56. DOI: 10.1016/s1286-4579(03)00049-2. View

19.

Nagamine K, Hase T, Notomi T . Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes. 2002; 16(3):223-9. DOI: 10.1006/mcpr.2002.0415. View

20.

Misawa Y, Yoshida A, Saito R, Yoshida H, Okuzumi K, Ito N . Application of loop-mediated isothermal amplification technique to rapid and direct detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood cultures. J Infect Chemother. 2007; 13(3):134-40. DOI: 10.1007/s10156-007-0508-9. View