Sensitive and Rapid Detection of Shigella and Enteroinvasive Escherichia Coli by a Loop-mediated Isothermal Amplification Method

Overview

Journal FEMS Microbiol Lett

Publisher Oxford University Press

Specialty Microbiology

Date 2005 Jan 26

PMID 15668027

Citations 33

Authors

Tianyan Song

Claudia Toma

Noboru Nakasone

Masaaki Iwanaga

Affiliations

Soon will be listed here.

Abstract

Here we report a loop-mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli. The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction.

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