Detection of Clostridium Perfringens Type A Enterotoxin in Faecal and Food Samples Using Immunomagnetic Separation (IMS)-ELISA

Overview

Journal Int J Food Microbiol

Specialties Microbiology
Nutritional Sciences

Date 1991 Apr 1

PMID 1854600

Citations 8

Authors

K S Cudjoe

L I Thorsen

T Sorensen

J Reseland

O Olsvik

P E Granum

Affiliations

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Abstract

A simple, rapid and sensitive immunoassay, based on immunomagnetic particles (Dynabeads M-280) was developed for detection and quantitation of Clostridium perfringens type A enterotoxin from faecal and food extracts. The assay had a detection limit of 2.5 ng/ml enterotoxin in homogenates of faeces and inoculated meat extracts. The specificity was confirmed by both crossed immunoelectrophoresis and Western immunoblotting techniques, using a purified enterotoxin as standard.

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