Protein Expression Changes in Maize Roots in Response to Humic Substances

Overview

Journal J Chem Ecol

Publisher Springer

Specialties Chemistry
Environmental Health

Date 2008 Jun 4

PMID 18521681

Citations 10

Authors

Paolo Carletti

Antonio Masi

Barbara Spolaore

Patrizia Polverino de Laureto

Mariangela De Zorzi

Loris Turetta

Massimo Ferretti

Serenella Nardi

Affiliations

Soon will be listed here.

Abstract

Humic substances are known to affect plant metabolism at different levels. We characterized humic substances extracted from earthworm feces by diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy and used them to treat corn, Zea mays L., seedlings to investigate changes in patterns of root protein expression. After root plasma membrane extraction and purification, proteins were separated by two-dimensional gel electrophoresis, and differential spot intensities were evaluated by image analysis. Finally, 42 differentially expressed proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The majority of them were downregulated by the treatment with humic substances. The proteins identified included malate dehydrogenase, ATPases, cytoskeleton proteins, and different enzymes belonging to the glycolytic/gluconeogenic pathways and sucrose metabolism. The identification of factors involved in plant responses to humic substances may improve our understanding of plant-soil cross-talk, and enable a better management of soil resources.

Citing Articles

Analysis of Plant Physiological Parameters and Gene Transcriptional Changes Under the Influence of Humic Acid and Humic Acid-Amino Acid Combinations in Maize.

Decsi K, Ahmed M, Rizk R, Abdul-Hamid D, Toth Z Int J Mol Sci. 2025; 25(24.

PMID: 39769045 PMC: 11676358. DOI: 10.3390/ijms252413280.

Analysis of the molecular composition of humic substances and their effects on physiological metabolism in maize based on untargeted metabolomics.

Wang Y, Lu Y, Wang L, Song G, Ni L, Xu M Front Plant Sci. 2023; 14:1122621.

PMID: 37284724 PMC: 10239833. DOI: 10.3389/fpls.2023.1122621.

Evaluation of the effects of humic acids on maize root architecture by label-free proteomics analysis.

Nunes R, Domiciano G, Alves W, Melo A, Nogueira F, Canellas L Sci Rep. 2019; 9(1):12019.

PMID: 31427667 PMC: 6700139. DOI: 10.1038/s41598-019-48509-2.

Protein Profiling of Arabidopsis Roots Treated With Humic Substances: Insights Into the Metabolic and Interactome Networks.

Roomi S, Masi A, Conselvan G, Trevisan S, Quaggiotti S, Pivato M Front Plant Sci. 2019; 9:1812.

PMID: 30619394 PMC: 6299182. DOI: 10.3389/fpls.2018.01812.

Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.

Aguiar N, Olivares F, Novotny E, Canellas L PeerJ. 2018; 6:e5445.

PMID: 30202643 PMC: 6129145. DOI: 10.7717/peerj.5445.

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