Listeria Monocytogenes Biofilm-associated Protein (BapL) May Contribute to Surface Attachment of L. Monocytogenes but is Absent from Many Field Isolates

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2008 Jun 3

PMID 18515491

Citations 21

Authors

Suzanne J Jordan

Stefano Perni

Sarah Glenn

Isabel Fernandes

Manuela Barbosa

Manuela Sol

Rogerio P Tenreiro

Lelia Chambel

Belarmino Barata

Isabel Zilhao

Timothy G Aldsworth

Andreia Adriao

M Leonor Faleiro

Gilbert Shama

Peter W Andrew

Affiliations

Soon will be listed here.

Abstract

Listeria monocytogenes is a food-borne pathogen capable of adhering to a range of surfaces utilized within the food industry, including stainless steel. The factors required for the attachment of this ubiquitous organism to abiotic surfaces are still relatively unknown. In silico analysis of the L. monocytogenes EGD genome identified a putative cell wall-anchored protein (Lmo0435 [BapL]), which had similarity to proteins involved in biofilm formation by staphylococci. An insertion mutation was constructed in L. monocytogenes to determine the influence of this protein on attachment to abiotic surfaces. The results show that the protein may contribute to the surface adherence of strains that possess BapL, but it is not an essential requirement for all L. monocytogenes strains. Several BapL-negative field isolates demonstrated an ability to adhere to abiotic surfaces equivalent to that of BapL-positive strains. BapL is not required for the virulence of L. monocytogenes in mice.

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