Herpes Simplex Virus Type 1 Long-term Persistence, Latency, and Reactivation in Infected Burkitt Lymphoma Cells

Overview

Journal Arch Virol

Specialty Microbiology

Date 1991 Jan 1

PMID 1850231

Citations 2

Authors

W Hampl

S Conrad

A K KLEINSCHMIDT

Affiliations

Soon will be listed here.

Abstract

The two herpes simplex virus type 1 (HSV-1) strains F and AK which differ in virus-cell interaction and in DNA organization, were used to establish persistently productive infections in Burkitt lymphoma-derived cell lines BJAB and Raji. Four such lines could be maintained over a period of three years. Like the uninfected parental lines, the persistently infected cells display a cyclic pattern of cell proliferation. The expression of HSV-1-specific antigens proved to be variable. As a consequence, virus yields also vary within a subcultivation period. Pooled human HSV antisera, when continuously present, suppress virus production (inducible latency) and support cell proliferation to higher rates. By contrast, removal of the antiserum after a certain period of cultivation leads to virus reactivation with a delay of 8 to 20 days. After cultivation periods of more than 3 to 12 weeks, replacement of HSV antiserum does no longer result in virus reactivation and even inducers fail to reactivate.

Citing Articles

The role of the immune system in establishment of herpes simplex virus latency--studies using CD4+ T-cell depleted mice.

Schmidt D, Eis-Hubinger A, SCHNEWEIS K Arch Virol. 1993; 133(1-2):179-87.

PMID: 8240008 DOI: 10.1007/BF01309753.

HSV type 1 genome variants from persistently productive infections in Raji and BJAB cell lines.

Klauck S, Hampl W, KLEINSCHMIDT A Arch Virol. 1995; 140(7):1195-213.

PMID: 7646352 DOI: 10.1007/BF01322746.

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