The Amino-terminal Half of Rotavirus SA114fM VP4 Protein Contains a Hemagglutination Domain and Primes for Neutralizing Antibodies to the Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1991 Mar 1

PMID 1847459

Citations 22

Authors

M Lizano

S Lopez

C F Arias

Affiliations

Soon will be listed here.

Abstract

We have previously reported the synthesis in Escherichia coli of polypeptide MS2-VP8', which contains the amino-terminal half of the SA114fM VP4 protein fused to MS2 bacteriophage polymerase sequences (C. F. Arias, M. Lizano, and S. López, J. Gen. Virol. 68:633-642, 1987). In this work we have synthesized the carboxy-terminal half of the VP4 protein also fused to the MS2 polymerase. This protein, designated MS2-VP5', was recognized by sera to the complete virion and was able to induce antibodies to the virus when administered to mice; however, these antibodies had no neutralizing activity. The two chimeric polypeptides were tested for their ability to agglutinate erythrocytes and to prime the immune system of mice. Bacterial lysates enriched for the MS2-VP8' hybrid polypeptide, but not those enriched for the MS2-VP5' protein or those containing proteins from the host E. coli strain, had hemagglutinating activity. This hemagglutination was inhibited by sera to SA114fM rotavirus. In addition, a single dose of the MS2-VP8' polypeptide was able to prime the immune system of mice for an augmented neutralizing antibody response when the animals were subsequently immunized with purified SA114fM virus.

Citing Articles

Role of sialic acids in rotavirus infection.

Isa P, Arias C, Lopez S Glycoconj J. 2006; 23(1-2):27-37.

PMID: 16575520 PMC: 7087688. DOI: 10.1007/s10719-006-5435-y.

Characterization of neuraminidase-resistant mutants derived from rotavirus porcine strain OSU.

Lopez J, Maldonado A, Gerder M, Abanero J, Murgich J, Pujol F J Virol. 2005; 79(16):10369-75.

PMID: 16051829 PMC: 1182648. DOI: 10.1128/JVI.79.16.10369-10375.2005.

pH-induced conformational change of the rotavirus VP4 spike: implications for cell entry and antibody neutralization.

Pesavento J, Crawford S, Roberts E, Estes M, Prasad B J Virol. 2005; 79(13):8572-80.

PMID: 15956598 PMC: 1143764. DOI: 10.1128/JVI.79.13.8572-8580.2005.

RNA silencing of rotavirus gene expression.

Arias C, Dector M, Segovia L, Lopez T, Camacho M, Isa P Virus Res. 2004; 102(1):43-51.

PMID: 15068879 PMC: 7126860. DOI: 10.1016/j.virusres.2004.01.014.

Gene expression pattern in Caco-2 cells following rotavirus infection.

Cuadras M, Feigelstock D, An S, Greenberg H J Virol. 2002; 76(9):4467-82.

PMID: 11932413 PMC: 155077. DOI: 10.1128/jvi.76.9.4467-4482.2002.

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