Regulation of Polyphosphoinositide-specific Phospholipase C Activity by Purified Gq

Overview

Journal Science

Specialty Science

Date 1991 Feb 15

PMID 1846707

Citations 195

Authors

A V Smrcka

J R Hepler

K O Brown

P C Sternweis

Affiliations

Soon will be listed here.

Abstract

The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) by phospholipase C yields the second messengers inositol 1,4,5-trisphosphate (InsP3) and 1,2-diacylglycerol. This activity is regulated by a variety of hormones through G protein pathways. However, the specific G protein or proteins involved has not been identified. The alpha subunit of a newly discovered pertussis toxin-insensitive G protein (Gq) has recently been isolated and is now shown to stimulate the activity of polyphosphoinositide-specific phospholipase C (PI-PLC) from bovine brain. Both the maximal activity and the affinity of PI-PLC for calcium ion were affected. These results identify Gq as a G protein that regulates PI-PLC.

Citing Articles

A adenosine receptor-triggered intracellular calcium mobilization: Cell type-dependent involvement of G, G, G proteins and protein kinase C.

Gao Z, Gao R, Meyer C, Jacobson K Purinergic Signal. 2025; .

PMID: 39934472 DOI: 10.1007/s11302-025-10070-1.

A2B adenosine receptor-triggered intracellular calcium mobilization: Cell type-dependent involvement of Gi, Gq, Gs proteins and protein kinase C.

Gao Z, Gao R, Meyer C, Jacobson K Res Sq. 2024; .

PMID: 39711556 PMC: 11661376. DOI: 10.21203/rs.3.rs-5442142/v1.

G protein-coupled receptors: a gateway to targeting oncogenic EVs?.

Di Niro L, Linders A, Glynn T, Pegtel D, Siderius M, Crudden C Extracell Vesicles Circ Nucl Acids. 2024; 5(2):233-248.

PMID: 39698539 PMC: 11648488. DOI: 10.20517/evcna.2024.10.

A molecular mechanism to diversify Ca signaling downstream of Gs protein-coupled receptors.

Brands J, Bravo S, Jurgenliemke L, Gratz L, Schihada H, Frechen F Nat Commun. 2024; 15(1):7684.

PMID: 39227390 PMC: 11372221. DOI: 10.1038/s41467-024-51991-6.

GPCR-Gα13 Involvement in Mitochondrial Function, Oxidative Stress, and Prostate Cancer.

Wu D, Casey P Int J Mol Sci. 2024; 25(13).

PMID: 39000269 PMC: 11241654. DOI: 10.3390/ijms25137162.