Differential Contribution of Cardiac Sarcomeric Proteins in the Myofibrillar Force Response to Stretch

Overview

Journal Pflugers Arch

Specialty Physiology

Date 2008 May 2

PMID 18449562

Citations 29

Authors

Younss Ait Mou

Jean-Yves Le Guennec

Emilio Mosca

Pieter P de Tombe

Olivier Cazorla

Affiliations

Soon will be listed here.

Abstract

The present study examined the contribution of myofilament contractile proteins to regional function in guinea pig myocardium. We investigated the effect of stretch on myofilament contractile proteins, Ca(2+) sensitivity, and cross-bridge cycling kinetics (K (tr)) of force in single skinned cardiomyocytes isolated from the sub-endocardial (ENDO) or sub-epicardial (EPI) layer. As observed in other species, ENDO cells were stiffer, and Ca(2+) sensitivity of force at long sarcomere length was higher compared with EPI cells. Maximal K (tr) was unchanged by stretch, but was higher in EPI cells possibly due to a higher alpha-MHC content. Submaximal Ca(2+)-activated K (tr) increased only in ENDO cells with stretch. Stretch of skinned ENDO muscle strips induced increased phosphorylation in both myosin-binding protein C and myosin light chain 2. We concluded that transmural MHC isoform expression and differential regulatory protein phosphorylation by stretch contributes to regional differences in stretch modulation of activation in guinea pig left ventricle.

Citing Articles

Contractile State Dependent Sarcomere Length Variability in Isolated Guinea-Pig Cardiomyocytes.

Lookin O, Khokhlova A, Myachina T, Butova X, Cazorla O, de Tombe P Front Physiol. 2022; 13:857471.

PMID: 35444559 PMC: 9013801. DOI: 10.3389/fphys.2022.857471.

Novel insights into sarcomere regulatory systems control of cardiac thin filament activation.

Solis C, Solaro R J Gen Physiol. 2021; 153(7).

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High efficiency preparation of skinned mouse cardiac muscle strips from cryosections for contractility studies.

Feng H, Jin J Exp Physiol. 2020; 105(11):1869-1881.

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The Effects of Mechanical Preload on Transmural Differences in Mechano-Calcium-Electric Feedback in Single Cardiomyocytes: Experiments and Mathematical Models.

Khokhlova A, Konovalov P, Iribe G, Solovyova O, Katsnelson L Front Physiol. 2020; 11:171.

PMID: 32256377 PMC: 7091561. DOI: 10.3389/fphys.2020.00171.

Length-Dependent Activation of Contractility and Ca-Transient Kinetics in Auxotonically Contracting Isolated Rat Ventricular Cardiomyocytes.

Lookin O, Protsenko Y Front Physiol. 2020; 10:1473.

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