Role of SiRNA Silencing of MMP-2 Gene on Invasion and Growth of Laryngeal Squamous Cell Carcinoma

Overview

Journal Eur Arch Otorhinolaryngol

Specialty Otorhinolaryngology

Date 2008 Apr 26

PMID 18437407

Citations 22

Authors

Yanan Sun

Ming Liu

Baofeng Yang

Baoxin Li

Jianguang Lu

Affiliations

Soon will be listed here.

Abstract

The purpose of this study was to explore the effects of MMP-2 silencing on the invasion and growth of laryngeal squamous cell carcinoma (LSCC). Hep-2 cells were transfected with MMP-2-RNAi-Lentivirus, and MMP-2 expression and invasive properties of the cells were evaluated. The experimental tumors in the nude mice were intratumorally injected with the same recombinant lentivirus. The inhibition of tumor growth was observed. The expression of MMP-2 protein in MMP-2 siRNA transfected Hep-2 cells was effectively suppressed. Both the viability and invasive ability of Hep-2 cells were significantly inhibited. The average weight and volume of tumor in MMP-2-RNAi-Lentivirus treated group were significantly lower than those in the control group (P < 0.01). The PCNA index was obviously lower in MMP-2 RNAi treated tumors (P < 0.01). In conclusion, MMP-2 silencing by recombinant lentivirus mediated RNA interference can inhibit invasion and growth of LSCC, and MMP-2 might be a potential target for gene therapy in LSCC.

Citing Articles

WDR12/RAC1 axis promoted proliferation and anti-apoptosis in colorectal cancer cells.

Wen S, Huang X, Xiong L, Zeng H, Wu S, An K Mol Cell Biochem. 2024; 479(12):3341-3354.

PMID: 38341833 DOI: 10.1007/s11010-024-04937-x.

Acts as an Oncogene in Bladder Cancer.

Huang N, Peng L, Yang J, Li J, Zhang S, Sun M Cancers (Basel). 2023; 15(21).

PMID: 37958297 PMC: 10648174. DOI: 10.3390/cancers15215122.

Silencing RNA for MMPs May Be Utilized for Cardioprotection.

Banaszkiewicz M, Krzywonos-Zawadzka A, Olejnik A, Noszczyk-Nowak A, Bil-Lula I Cardiovasc Ther. 2022; 2022:9729018.

PMID: 36082193 PMC: 9433229. DOI: 10.1155/2022/9729018.

Evaluation of calcium-binding protein A11 promotes the carcinogenesis of hypopharygeal squamous cell carcinoma via the PI3K/AKT signaling pathway.

Wang C, Lin C, Tao Q, Zhao S, Liu H, Li L Am J Transl Res. 2019; 11(6):3472-3480.

PMID: 31312359 PMC: 6614622.

Identification of gene expression models for laryngeal squamous cell carcinoma using co-expression network analysis.

Yang C, Wang S, Yang X, Wang L, Niu L, Liu J Medicine (Baltimore). 2018; 97(7):e9738.

PMID: 29443735 PMC: 5839854. DOI: 10.1097/MD.0000000000009738.

References

Pacheco M, Mourao M, Mantovani E, Nishimoto I, Brentani M . Expression of gelatinases A and B, stromelysin-3 and matrilysin genes in breast carcinomas: clinico-pathological correlations. Clin Exp Metastasis. 1999; 16(7):577-85. DOI: 10.1023/a:1006580415796. View

Folgueras A, Pendas A, Sanchez L, Lopez-Otin C . Matrix metalloproteinases in cancer: from new functions to improved inhibition strategies. Int J Dev Biol. 2004; 48(5-6):411-24. DOI: 10.1387/ijdb.041811af. View

Chetty C, Bhoopathi P, Lakka S, Rao J . MMP-2 siRNA induced Fas/CD95-mediated extrinsic II apoptotic pathway in the A549 lung adenocarcinoma cell line. Oncogene. 2007; 26(55):7675-83. PMC: 2167629. DOI: 10.1038/sj.onc.1210584. View

Gohji K, Fujimoto N, Fujii A, Komiyama T, Okawa J, Nakajima M . Prognostic significance of circulating matrix metalloproteinase-2 to tissue inhibitor of metalloproteinases-2 ratio in recurrence of urothelial cancer after complete resection. Cancer Res. 1996; 56(14):3196-8. View

Liu M, Lawson G, Delos M, Jamart J, Chatelain B, Remacle M . Prognostic value of cell proliferation markers, tumour suppressor proteins and cell adhesion molecules in primary squamous cell carcinoma of the larynx and hypopharynx. Eur Arch Otorhinolaryngol. 2003; 260(1):28-34. DOI: 10.1007/s00405-002-0512-8. View

Kariya Y, Miyazaki K . The basement membrane protein laminin-5 acts as a soluble cell motility factor. Exp Cell Res. 2004; 297(2):508-20. DOI: 10.1016/j.yexcr.2004.03.044. View

Xie M, Sun Y, Li Y . Expression of matrix metalloproteinases in supraglottic carcinoma and its clinical implication for estimating lymph node metastases. Laryngoscope. 2004; 114(12):2243-8. DOI: 10.1097/01.mlg.0000149467.18822.59. View

Koshikawa N, Schenk S, Moeckel G, Sharabi A, Miyazaki K, Gardner H . Proteolytic processing of laminin-5 by MT1-MMP in tissues and its effects on epithelial cell morphology. FASEB J. 2003; 18(2):364-6. DOI: 10.1096/fj.03-0584fje. View

Fire A, Xu S, Montgomery M, Kostas S, Driver S, Mello C . Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998; 391(6669):806-11. DOI: 10.1038/35888. View

10.

Ikebe T, Shinohara M, Takeuchi H, Beppu M, Kurahara S, Nakamura S . Gelatinolytic activity of matrix metalloproteinase in tumor tissues correlates with the invasiveness of oral cancer. Clin Exp Metastasis. 1999; 17(4):315-23. DOI: 10.1023/a:1006642428826. View

11.

Egeblad M, Werb Z . New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer. 2002; 2(3):161-74. DOI: 10.1038/nrc745. View

12.

Gu W, Putral L, Hengst K, Minto K, Saunders N, Leggatt G . Inhibition of cervical cancer cell growth in vitro and in vivo with lentiviral-vector delivered short hairpin RNA targeting human papillomavirus E6 and E7 oncogenes. Cancer Gene Ther. 2006; 13(11):1023-32. DOI: 10.1038/sj.cgt.7700971. View

13.

Overall C, Lopez-Otin C . Strategies for MMP inhibition in cancer: innovations for the post-trial era. Nat Rev Cancer. 2002; 2(9):657-72. DOI: 10.1038/nrc884. View

14.

Brakebusch C, Bouvard D, Stanchi F, Sakai T, Fassler R . Integrins in invasive growth. J Clin Invest. 2002; 109(8):999-1006. PMC: 150953. DOI: 10.1172/JCI15468. View

15.

McCawley L, Matrisian L . Matrix metalloproteinases: they're not just for matrix anymore!. Curr Opin Cell Biol. 2001; 13(5):534-40. DOI: 10.1016/s0955-0674(00)00248-9. View

16.

Liu W, Zeng Z, Wu Q, Hou J, Chen Y . Overexpression of MMP-2 in laryngeal squamous cell carcinoma: a potential indicator for poor prognosis. Otolaryngol Head Neck Surg. 2005; 132(3):395-400. DOI: 10.1016/j.otohns.2004.09.050. View

17.

Naldini L, Blomer U, Gallay P, Ory D, Mulligan R, Gage F . In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science. 1996; 272(5259):263-7. DOI: 10.1126/science.272.5259.263. View

18.

Noel A, Maillard C, Rocks N, Jost M, Chabottaux V, Sounni N . Membrane associated proteases and their inhibitors in tumour angiogenesis. J Clin Pathol. 2004; 57(6):577-84. PMC: 1770325. DOI: 10.1136/jcp.2003.014472. View

19.

Sumimoto H, Hirata K, Yamagata S, Miyoshi H, Miyagishi M, Taira K . Effective inhibition of cell growth and invasion of melanoma by combined suppression of BRAF (V599E) and Skp2 with lentiviral RNAi. Int J Cancer. 2005; 118(2):472-6. DOI: 10.1002/ijc.21286. View

20.

Davidson B, Goldberg I, Gotlieb W, Kopolovic J, Ben-Baruch G, Nesland J . High levels of MMP-2, MMP-9, MT1-MMP and TIMP-2 mRNA correlate with poor survival in ovarian carcinoma. Clin Exp Metastasis. 2000; 17(10):799-808. DOI: 10.1023/a:1006723011835. View