Differentiating Proteomic Biomarkers in Breast Cancer by Laser Capture Microdissection and MALDI MS

Overview

Journal J Proteome Res

Publisher American Chemical Society

Specialty Biochemistry

Date 2008 Apr 5

PMID 18386930

Citations 31

Authors

Melinda E Sanders

Eduardo C Dias

Baogang J Xu

James A Mobley

Dean Billheimer

Heinrich Roder

Julia Grigorieva

Mitchell Dowsett

Carlos L Arteaga

Richard M Caprioli

Affiliations

Soon will be listed here.

Abstract

We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identified by statistical analysis and classifiers were developed and validated in blinded test sets. Several of the m/ z features used in the classifiers were identified by LC-MS/MS and two were confirmed by immunohistochemistry.

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