Platelet Counts and Platelet Activation Markers in Obese Subjects

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2008 Apr 4

PMID 18385810

Citations 57

Authors

Dorit Samocha-Bonet

Dan Justo

Ori Rogowski

Nili Saar

Subchi Abu-Abeid

Galina Shenkerman

Itzhak Shapira

Shlomo Berliner

Aaron Tomer

Affiliations

Soon will be listed here.

Abstract

Objective: In this work we studied the correlation between platelet count, platelet activation, and systemic inflammation in overweight, obese, and morbidly obese individuals.

Methods And Subjects: A total of 6319 individuals participated in the study. Complete blood counts, high sensitivity C-reactive protein (hs-CRP) serum levels, and body mass index (BMI) were measured during routine checkups. Platelet activation markers were studied among 30 obese (BMI = 41 +/- 8 kg/m(2)) and 35 nonobese (BMI = 24 +/- 3 kg/m(2)) individuals. Platelet activation status was evaluated by flow cytometry using specific antibodies against the activated platelet membrane glycoprotein IIb/IIIa, p-selectin (CD-62 p), and binding of Annexin-V to platelet anionic phospholipids.

Results: Overweight, obese, and morbidly obese females had significantly elevated platelet counts ( P < .0001) compared with normal-weight females. No significant elevation of platelet counts was observed in the male subgroups. A significant age adjusted correlation between BMI and platelet counts ( P < .0001) was found among females. This correlation was attenuated (P = .001) after adjustment for hs-CRP concentrations. The flow cytometry analysis of platelets showed no significant differences in activation marker expression between nonobese and obese individuals.

Discussion: Obesity may be associated with elevated platelet counts in females with chronic inflammation. Obesity is not associated with increased platelet activation.

Citing Articles

Effect of age, sex, breed and venipuncture site on platelet count and clumping in feline blood samples.

Larkin S, Kornya M J Feline Med Surg. 2025; 27(1):1098612X241305919.

PMID: 39815974 PMC: 11736786. DOI: 10.1177/1098612X241305919.

Unexpected cardiovascular risks of glucagon-like peptide-1 receptor agonist and aspirin co-administration in individuals with obesity, with and without type 2 diabetes: A propensity score matched cohort study.

Lin C, Chen J, Meyerowitz-Katz G, Huang Y, Su P Diabetes Obes Metab. 2025; 27(4):1980-1991.

PMID: 39806559 PMC: 11885080. DOI: 10.1111/dom.16191.

The impact of a short-term high-fat diet on coagulation function in a mouse model and its role in exacerbating concanavalin A-induced liver injury.

Nanizawa E, Tamaki Y, Yakura T, Otsuka S, Hatayama N, Naito M BMC Nutr. 2024; 10(1):158.

PMID: 39696700 PMC: 11654324. DOI: 10.1186/s40795-024-00966-3.

Effects of exercise on cAMP-mediated platelet inhibition in young women: a pilot study.

ODonoghue L, Crognale D, Delahunt E, Smolenski A Eur J Appl Physiol. 2024; .

PMID: 39636435 DOI: 10.1007/s00421-024-05673-2.

Platelet and white blood cell counts correlate with leptin and body mass index in Japanese adolescents.

Takaya J, Tanabe Y, Nomura N, Minami M, Onuma C, Yamagishi M Clin Pediatr Endocrinol. 2024; 33(4):207-213.

PMID: 39359671 PMC: 11442701. DOI: 10.1297/cpe.2024-0045.

References

Dogru T, Tasci I, Sonmez A, Genc H, Gok M, Yilmaz M . The plasma levels of soluble P-selectin in subjects with prediabetes. Int J Clin Pract. 2006; 60(9):1048-52. DOI: 10.1111/j.1742-1241.2006.00969.x. View

Williams J, Pipia G, Datta N, Long M . Thrombopoietin requires additional megakaryocyte-active cytokines for optimal ex vivo expansion of megakaryocyte precursor cells. Blood. 1998; 91(11):4118-26. View

Collins C, Rampton D . Review article: platelets in inflammatory bowel disease--pathogenetic role and therapeutic implications. Aliment Pharmacol Ther. 1997; 11(2):237-47. DOI: 10.1046/j.1365-2036.1997.153328000.x. View

Eckel R, Krauss R . American Heart Association call to action: obesity as a major risk factor for coronary heart disease. AHA Nutrition Committee. Circulation. 1998; 97(21):2099-100. DOI: 10.1161/01.cir.97.21.2099. View

Fabris F, Randi M, Casonato A, Dal Bo Zanon R, Bonvicini P, Girolami A . Clinical significance of beta-thromboglobulin in patients with high platelet count. Acta Haematol. 1984; 71(1):32-8. DOI: 10.1159/000206503. View

Scharf R, Tomer A, Marzec U, Teirstein P, Ruggeri Z, Harker L . Activation of platelets in blood perfusing angioplasty-damaged coronary arteries. Flow cytometric detection. Arterioscler Thromb. 1992; 12(12):1475-87. DOI: 10.1161/01.atv.12.12.1475. View

Carter A, Hanley S . The effect of platelet number and haematocrit on whole blood thromboxane synthesis. Thromb Haemost. 1985; 53(2):225-7. View

Yudkin J, Kumari M, Humphries S, Mohamed-Ali V . Inflammation, obesity, stress and coronary heart disease: is interleukin-6 the link?. Atherosclerosis. 2000; 148(2):209-14. DOI: 10.1016/s0021-9150(99)00463-3. View

Grundy S, Brewer Jr H, Cleeman J, Smith Jr S, Lenfant C . Definition of metabolic syndrome: Report of the National Heart, Lung, and Blood Institute/American Heart Association conference on scientific issues related to definition. Circulation. 2004; 109(3):433-8. DOI: 10.1161/01.CIR.0000111245.75752.C6. View

10.

Yudkin J, Stehouwer C, Emeis J, Coppack S . C-reactive protein in healthy subjects: associations with obesity, insulin resistance, and endothelial dysfunction: a potential role for cytokines originating from adipose tissue?. Arterioscler Thromb Vasc Biol. 1999; 19(4):972-8. DOI: 10.1161/01.atv.19.4.972. View

11.

Nakata M, Yada T, Soejima N, Maruyama I . Leptin promotes aggregation of human platelets via the long form of its receptor. Diabetes. 1999; 48(2):426-9. DOI: 10.2337/diabetes.48.2.426. View

12.

De Pergola G, Pannacciulli N, Coviello M, Scarangella A, Di Roma P, Caringella M . sP-selectin plasma levels in obesity: association with insulin resistance and related metabolic and prothrombotic factors. Nutr Metab Cardiovasc Dis. 2007; 18(3):227-32. DOI: 10.1016/j.numecd.2006.09.010. View

13.

Licata G, Scaglione R, Giammarresi C, Davi G . No evidence of platelet activation in obesity. Thromb Haemost. 1993; 70(3):544. View

14.

Rifai N, Tracy R, Ridker P . Clinical efficacy of an automated high-sensitivity C-reactive protein assay. Clin Chem. 1999; 45(12):2136-41. View

15.

Konstantinides S, Schafer K, Koschnick S, Loskutoff D . Leptin-dependent platelet aggregation and arterial thrombosis suggests a mechanism for atherothrombotic disease in obesity. J Clin Invest. 2001; 108(10):1533-40. PMC: 209418. DOI: 10.1172/JCI13143. View

16.

Baatout S . Interleukin-6 and megakaryocytopoiesis: an update. Ann Hematol. 1996; 73(4):157-62. DOI: 10.1007/s002770050220. View

17.

Ly H, Kirtane A, Murphy S, Buros J, Cannon C, Braunwald E . Association of platelet counts on presentation and clinical outcomes in ST-elevation myocardial infarction (from the TIMI Trials). Am J Cardiol. 2006; 98(1):1-5. DOI: 10.1016/j.amjcard.2006.01.046. View

18.

Nieto F, Szklo M, Folsom A, Rock R, Mercuri M . Leukocyte count correlates in middle-aged adults: the Atherosclerosis Risk in Communities (ARIC) Study. Am J Epidemiol. 1992; 136(5):525-37. DOI: 10.1093/oxfordjournals.aje.a116530. View

19.

McCabe D, Harrison P, Mackie I, Sidhu P, Purdy G, Lawrie A . Increased platelet count and leucocyte-platelet complex formation in acute symptomatic compared with asymptomatic severe carotid stenosis. J Neurol Neurosurg Psychiatry. 2005; 76(9):1249-54. PMC: 1739812. DOI: 10.1136/jnnp.2004.051003. View

20.

Lazzari L, Henschler R, Lecchi L, Rebulla P, Mertelsmann R, Sirchia G . Interleukin-6 and interleukin-11 act synergistically with thrombopoietin and stem cell factor to modulate ex vivo expansion of human CD41+ and CD61+ megakaryocytic cells. Haematologica. 2000; 85(1):25-30. View